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Transcription in Prokaryotes

Total questions: 10

Worksheet time: 7mins

Name
Class
Date
1.

How does coupling of transcription and translation in prokaryotes differ from eukaryotes?

a)

In prokaryotes, transcription and translation occur simultaneously in the same cellular compartment

b)

In prokaryotes, transcription and translation occur in separate cellular compartments

c)

In prokaryotes, multiple rounds of transcription must be completed before translation begins

d)

In prokaryotes, translation begins only after the transcription termination

2.

Which subunit of prokaryotic RNA polymerase is responsible for promoter recognition?

a)

β subunit

b)

ω (omega) subunit

c)

σ (sigma) subunit

d)

α subunit

3.

What does the following strand represent?

3' TGCATGCCTACGATTTAAGGCGTGAC 5'

a)

DNA Coding Strand

b)

Template Strand

c)

mRNA starnd

d)

Non-template strand

4.

Which of the following describes the consensus sequence of the -10 box (Pribnow box)

in prokaryotic promoters?

a)

AATAAA

b)

TACAAT

c)

TTGACA

d)

TATAAT

5.

What is the primary function of the NusA protein in prokaryotic transcription?

a)

It catalyzes the addition of nucleotides to the RNA chain

b)

It recognizes promoter sequences

c)

It enhances termination at intrinsic terminators

d)

It prevents backtracking of RNA polymerase

6.

Which element defines the exact starting point of transcription in a prokaryotic promoter?

a)

The -10 box (Pribnow box)

b)

The -35 box

c)

The transcription factor binding site

d)

The +1 site

7.

What happens to the sigma (σ) factor during the transition from initiation to elongation

in prokaryotic transcription?

a)

It remains bound to RNA polymerase throughout transcription

b)

It dissociates from the core enzyme

c)

It is degraded by cellular proteases

d)

It changes conformation to facilitate elongation

8.

What is the UP element in a prokaryotic promoter?

a)

A sequence downstream of the transcription start site

b)

A sequence upstream of the -35 element that binds the α subunit of RNA polymerase

c)

A sequence between the -10 and -35 elements

d)

A sequence involved in termination

9.

What advantage does the coupling of transcription and translation in prokaryotes provide?

a)

It provides immediate production of proteins and efficient resource utilization

b)

It increases the accuracy of protein synthesis

c)

It prevents the formation of RNA secondary structures

d)

It allows for more complex gene regulation

10.

Which of the following is NOT involved in intrinsic (rho-independent) termination

of transcription in prokaryotes?

a)

ATP hydrolysis

b)

Palindromic sequence that forms a hairpin in the RNA

c)

Destabilization of the RNA-DNA hybrid

d)

A series of U residues in the RNA transcript