Distinct sequence of DNA which codes for a specific protein

Short DNA sequences that bind to specific parts of the target DNA sequence that tell the taq Polymerase where to start making a new strand of DNA

A DNA Polymerase found in bacteria that thrive around hot springs. It is used because it will not denature at high temperatures

A laboratory technique used to make billions of copies of a specific segment of DNA

A period of high temperature, 95°C, which unwinds the double-stranded DNA and creates two single-stranded DNA pieces. This is the first phase of the PCR Cycle

At a temperature of around 55^oC, DNA primers bind to the complementary sequences of the two single stranded DNA pieces. This is the second phase of the PCR Cycle.

At 75^oC, taq Polymerase binds to the primer and adds DNA nucleotides to each complementary single stranded DNA

The  process of DNA denaturation, primer annealing and nucleotide extension. Each cycle copies one DNA sequence and creates two identical DNA sequences