WorksheetsMIDTERMS Tricarboxylic Acid Cycle
Total questions: 25
Worksheet time: 25mins
Which of the following is the first compound formed in the TCA cycle?
Isocitrate
Citrate
Succinate
Fumarate
Which enzyme catalyzes the conversion of acetyl-CoA to citrate?
Isocitrate dehydrogenase
Alpha-ketoglutarate dehydrogenase
Citrate synthase
Aconitase
Which intermediate of the TCA cycle is directly involved in substrate-level phosphorylation?
Citrate
Fumarate
Succinyl-CoA
Malate
Which enzyme is responsible for the conversion of succinate to fumarate?
Succinate dehydrogenase
Fumarase
Malate dehydrogenase
Aconitase
The final product of the TCA cycle that is regenerated at the end of the cycle is:
Citrate
Oxaloacetate
Malate
Acetyl-CoA
Which of the following coenzymes is reduced during the conversion of isocitrate to alpha-ketoglutarate?
NAD+
FAD
CoA
ATP
Which molecule is produced during the decarboxylation of alpha-ketoglutarate?
ATP
NADH
CO₂
FADH₂
What is the final electron acceptor in the TCA cycle?
Oxygen
NAD+
FAD
Coenzyme Q
Which of the following is NOT a product of the TCA cycle?
NADH
ATP
FADH₂
Oxygen
What is the role of NADH and FADH₂ produced in the TCA cycle?
They are converted into ATP via substrate-level phosphorylation.
They carry electrons to the electron transport chain for ATP production.
They act as intermediates in the synthesis of glucose.
They regenerate oxaloacetate.
Which molecule is involved in the reaction catalyzed by aconitase?
Citric acid
Aconitate
Malate
Isocitrate
What happens during the reaction catalyzed by malate dehydrogenase in the TCA cycle?
Malate is converted to fumarate
Oxaloacetate is reduced to malate
Malate is oxidized to oxaloacetate
Fumarate is hydrated to form malate
Which of the following intermediates in the TCA cycle is involved in the biosynthesis of amino acids?
Citrate
Alpha-ketoglutarate
Fumarate
Succinyl-CoA
Which enzyme in the TCA cycle is regulated by ATP, NADH, and succinyl-CoA?
Alpha-ketoglutarate dehydrogenase
Citrate synthase
Malate dehydrogenase
Fumarase
How many NADH molecules are produced per round of the TCA cycle?
1
2
3
4
Which of the following enzymes catalyzes a reaction that produces FADH₂ in the TCA cycle?
Alpha-ketoglutarate dehydrogenase
Succinate dehydrogenase
Citrate synthase
Isocitrate dehydrogenase
What is the role of Coenzyme A in the TCA cycle?
It helps in the oxidation of acetyl-CoA.
It carries electrons to the electron transport chain.
It forms a bond with citrate.
It assists in the decarboxylation of alpha-ketoglutarate.
Which of the following molecules is produced during the conversion of succinyl-CoA to succinate?
FADH₂
ATP (or GTP)
NADH
CO₂
Which step in the TCA cycle is involved in generating GTP or ATP?
The conversion of succinate to fumarate
The conversion of succinyl-CoA to succinate
The conversion of isocitrate to alpha-ketoglutarate
The regeneration of oxaloacetate from malate
Which of the following is NOT a direct product of the TCA cycle?
ATP
NADH
Pyruvate
CO₂
Describe the role of the TCA cycle in cellular respiration. Include its connection to other metabolic pathways like glycolysis and fatty acid oxidation.
Explain the process by which acetyl-CoA enters the TCA cycle. What are the key steps involved in the formation of citrate?
Discuss the importance of the enzyme citrate synthase in regulating the TCA cycle. How is it regulated?
Outline the steps of the TCA cycle where NADH is produced. How does NADH contribute to energy production in the cell?
Describe the role of FADH₂ in the TCA cycle. How is FADH₂ generated, and what role does it play in ATP production?
