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DNA Analysis Techniques and Terminology

Total questions: 14

Worksheet time: 7mins

Name
Class
Date
1.
A laboratory technique used to determine the probable identity of an organism based on the nucleotide sequences of certain regions of DNA that are unique to individuals.
a)
Non-coding DNA
b)
DNA Ladder
c)
DNA Fingerprinting
d)
Migration
2.
Regions of a genome that do not code for proteins. Science used to think this DNA was just junk, but we are now learning the important purposes of non-coding regions of DNA.
a)
Band
b)
Gene
c)
Non-coding DNA
d)
DNA Ladder
3.
A protein that cuts DNA at specific sequences.
a)
Migration
b)
Genome
c)
Non-coding DNA
d)
Restriction enzyme
4.
Short Tandem Repeats. These are short repeating sequences of DNA that exist in non-coding regions of the genome.
a)
Agarose
b)
Wells
c)
STR's
d)
Lane
5.
A laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge. This technique allows us to visually analyze the size differences of the molecules in the samples.
a)
Restriction enzyme
b)
Genome
c)
Wells
d)
Gel Electrophoresis
6.
A sequence of DNA that codes for a protein. There are about 22,000 known genes in the human genome.
a)
Genome
b)
Lane
c)
Gene
d)
Band
7.
An organism's full set of DNA. There are 3 billion base pairs of DNA in the human genome.
a)
Agarose
b)
Lane
c)
Gene
d)
Genome
8.
A solution with charged, weak acids and bases that maintains a consistent pH. The charged atoms in the buffer conduct electricity through the gel.
a)
Band
b)
Gene
c)
Buffer
d)
Lane
9.
Where molecules in electrophoresis travel to the opposite side of the gel in a straight line.
a)
Genome
b)
Lane
c)
Gene
d)
Band
10.
A powder used to make a gel. It is purified from kelp (a type of seaweed).
a)
Genome
b)
STR's
c)
Gene
d)
Agarose
11.
Pockets in a gel where molecules are loaded.
a)
Gene
b)
STR's
c)
Wells
d)
Band
12.
Movement of molecules through a gel.
a)
Migration
b)
Lane
c)
STR's
d)
Agarose
13.
A line in a gel composed of billions of segments of DNA of the same length that traveled through the gel at the same speed.
a)
STR's
b)
Gene
c)
Lane
d)
Band
14.
A preparation of DNA fragments of known lengths added to one of the wells used to compare and estimate the length of the DNA samples.
a)
Lane
b)
Band
c)
Agarose
d)
DNA Ladder