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Protein Synthesis Week 3

Total questions: 25

Worksheet time: 38mins

Name
Class
Date
1.

Which of the following components is essential for translation?

a)

Spliceosome

b)

Ribosome

c)

DNA polymerase

d)

RNA polymerase

2.

tRNAs adapt specific triplet codons in mRNA to what?

a)

rRNAs

b)

Other codons

c)

Correct amino acids

d)

Exons

3.

Which part of the tRNA binds to the mRNA codon during translation?

a)

Acceptor stem

b)

D-loop

c)

Anticodon loop

d)

Variable loop

4.

The enzyme responsible for attaching amino acids to their corresponding tRNAs is called:

a)

Peptidyl transferase

b)

Aminoacyl tRNA synthetase

c)

DNA ligase

d)

Topoisomerase

5.

How many different types of aminoacyl tRNA synthetases exist in most organisms?

a)

64

b)

30-55

c)

20

d)

12

6.

Which site on the ribosome does the first charged tRNA bind during initiation?

a)

E site

b)

P site

c)

A site

d)

Exit tunnel

7.

What is the start codon for translation?

a)

UGA

b)

UAC

c)

AUG

d)

AAA

8.

During elongation, which site on the ribosome does the new tRNA enter?

a)

P site

b)

Exit tunnel

c)

A site

d)

D-loop

9.

What energy source drives elongation during translation?

a)

ATP

b)

NADH

c)

GTP

d)

FAD

10.

Which of the following codons signals termination of translation?

a)

AUG

b)

GGU

c)

UAA

d)

UAC

11.

What type of bond forms between amino acids during translation?

a)

Phosphodiester

b)

Peptide

c)

Glycosidic

d)

Ionic

12.

Which proteins mediate proper protein folding?

a)

Ligases

b)

Ubiquitins

c)

Chaperones

d)

Helicases

13.

Which molecule tags misfolded proteins for degradation?

a)

Protease

b)

Ubiquitin

c)

Chaperonin

d)

Ligase

14.

Where are misfolded proteins transported for degradation?

a)

Golgi apparatus

b)

Nucleus

c)

Proteasome

d)

Mitochondria

15.

Using the Genetic Code Chart, Please Translate the following -

5' - UGGCAAUAUCACAUGUUUCUUGGCUGA - 3' ?

a)

Phe-Leu-Gly

b)

Trp-Gln-Tyr-His-Met-Phe-Leu-Gly

c)

Val-Ser-Arg-Cys-Pro-Phe-Gly-Leu

d)

The above sequence cannot be translated due to the absence of Thymine in the DNA provided

16.

What determines the chemical nature of an amino acid?

a)

Carboxyl group

b)

Amino group

c)

R group

d)

Hydrogen atom

17.

Which class of amino acids is hydrophobic?

a)

Polar

b)

Nonpolar

c)

Positively charged

d)

Negatively charged

18.

Enzymes primarily function by:

a)

Storing genetic information

b)

Transporting mRNA

c)

Catalyzing chemical reactions

d)

Binding to ribosomes

19.

Using the Genetic Code Chart, which of the following statements would accurately describe the protein once translated using the mRNA sequence below?

5' - UGGCAAUAUCACAUGCUUUAAGGCUGA - 3'

a)

The protein will be truncated (shortened) due to an early stop code

b)

The protein will be comprised of 9 amino acids

c)

The protein will start with the amino acid, Trp

d)

It is impossible to know what the protein will be, given that the mRNA and not the DNA was provided.

20.

Which ribosomal subunit binds first to the mRNA during translation?

a)

Large Ribosomal Subunit

b)

Small Ribosomal Subunit

c)

Both bind at the same time

d)

Ribosomes are not involved in the initiation of translation

21.

What is one major difference between Translation and Transcription?

a)

In Translation we are making RNA from a DNA template, In Transcription we are creating DNA from an RNA template

b)

In Transcription we are making protein from a DNA template, while in Translation the final product is not clearly defined

c)

RNA Polymerase plays a major role in Translation and Transcription, but its function during translation is most critical to the process

d)

Translation involves the usage of mRNA, enzymes, and ribosomes, while transcription does not involve ribosomes

22.

Which process is shown?

a)

DNA Replication

b)

Transcription

c)

mRNA recombination

d)

Translation

23.

Which of the following processes involves the enzyme amino-acyl transferase?

a)

Translation

b)

DNA replication

c)

Transcription

d)

All Three Process involve this enzyme

24.

Which of the following process involves the use of DNA Polymerase III?

a)

The process of replication that occurs during S-Phase of the Cell Cycle

b)

The process of creating RNA from a DNA template

c)

The process of creating Protein from an mRNA template

d)

The process that involves the usage of ribosomes in the creation of amino acids

25.

Which of the following processes involves the use of TFIIA?

a)

Transcription

b)

Translation

c)

DNA Replication

d)

None of the Above