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Finals Unit 7 Review

Total questions: 18

Worksheet time: 18mins

Name
Class
Date
1.
What is recombinant DNA?
a)
Adding DNA from one organism into the DNA of another
b)
DNA which has been changed over generations by natural selection
c)
DNA that causes genetic disorders
d)
DNA that has been sequenced
2.

Which suspect left the blood stain?

a)

Bob

b)

Sue

c)

John

d)

Lisa

3.
DNA fingerprinting works because
a)
Most genes are dominant
b)
All organisms contain RNA
c)
The most important genes are different among most people
d)
No two people, except identical twins, have exactly the same DNA 
4.
Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?
a)
DNA is negatively charged so attracted to the positive end of the unit
b)
DNA is positively charged to attracted to the negative end of the unit
c)
the agarose gel in negatively charged
d)
the agarose gel is positively charged
5.
In gel electrophoresis, the largest DNA fragment will appear
a)
closest to the starting wells
b)
farthest from the starting wells
c)
three quarters away from the starting wells
d)
it depends on how many fragments there are
6.
Which is the primary purpose of using restriction enzymes in gel electrophoresis?
a)
It allows the strands of DNA to be cut into various lengths for testing
b)
It restricts the number of base pairs that can be tested in a sample
c)
It makes the testing simpler by moving the strands into the gel faster
d)
It charges the DNA strands
7.
The DNA fingerprint pattern of a child is
a)
75% similar to the father
b)
75% similar to the mother
c)
50% bands similar to father and rest similar to mother
d)
Exactly similar to that of both of the parents
8.

Mitochondrial DNA is inherited from the father.

a)

True

b)

False

9.

What is transformation?

a)

The use of viruses to transform or genetically


engineer cells.

b)

The measure of how well cells are transformed in a new phenotype.

c)

The insertion of a foreign plasmid into a bacterial cell which results in new acquired traits.

d)

Genetic engineering or transformation of mammalian cells.

10.

Cells are made competent by;

a)

The incubation of the bacteria at 95C

b)

A process that uses calcium chloride.

c)

A process that uses GFP

d)

A process that uses polymerases

11.

Heat shock

a)

Trap the plasmids inside the cell.

b)

Enlarges the pores of the cell.

c)

Provides for the death of the cell.

d)

Increases the disintegration of the cell wall.

12.
What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?
a)

ampicillin

b)
arabinose
c)
LB
d)
GFP
13.
Which gene is responsible for the bacteria glowing?
a)
bla
b)
araC
c)
GFP
d)
pGLO
14.
Which plate had bacterial growth and bacterial GLOWth?
a)
LB
b)
LB/Amp
c)
LB/Amp/Ara
d)
All
15.
 What was used to cut the plasmid to enable us to insert the desired gene?
a)
ligase
b)
restriction enzymes
c)
GFP
d)
pGLO
16.
Which gene is responsible for making the bacteria resistant to ampicillin?
a)

ampR

b)
pGLO
c)
araC
d)
GFP
17.

What is the name of the plasmid we used TO TRANSORM THE BACTERIA?

a)

GFP

b)

bla

c)

araC

d)

pGLO

18.

What is recombinant DNA?

a)

DNA from one organism added to another

b)

DNA from 2 different ribosomes

c)

DNA that is combined through selective breeding