Wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

Alginate Immobilized Amylase Quiz

Total questions: 10

Worksheet time: 5mins

Name
Class
Date
1.

Which of the following best explains the reason for immobilizing amylase in calcium alginate beads?

a)

To change the pH of the enzyme

b)

To increase enzyme inhibition

c)

To enhance the reusability and stability of the enzyme

d)

To denature the enzyme more quickly

2.

If an immobilized amylase bead breaks during a reaction, what is the most immediate impact on the experiment?

a)

Increased enzyme specificity

b)

Decrease in reaction pH

c)

Loss of enzyme from the reaction system

d)

Increase in bead strength

3.

A student immobilizes amylase using 3% sodium alginate and finds the reaction rate is too slow. What could they modify to potentially increase the rate?

a)

Increase the calcium chloride concentration slightly

b)

Increase the pH of the substrate solution to 11

c)

Use a larger bead size

d)

Decrease the substrate concentration

4.

Which condition would most likely reduce the effectiveness of alginate-immobilized amylase in hydrolyzing starch?

a)

Optimum pH and temperature maintained

b)

Use of high concentrations of calcium chloride

c)

Beads stored in acidic buffer for a long period

d)

Substrate concentration kept at saturating levels

5.

A researcher observes that free amylase has a higher reaction rate than immobilized amylase. Which factor could explain this?

a)

Enhanced substrate diffusion in immobilized form

b)

Enzyme activation by alginate matrix

c)

Mass transfer limitations in immobilized systems

d)

Faster removal of product from immobilized enzyme

6.

You are tasked to design an experiment comparing free and immobilized amylase. What would be an appropriate control variable?

a)

Type of gel used

b)

Concentration of substrate used

c)

pH of sodium alginate

d)

Bead color

7.

In an industrial setup, why might alginate immobilization be preferred for amylase in continuous starch hydrolysis?

a)

It increases the cost of enzyme usage

b)

It prevents enzyme reuse

c)

It allows easy separation of enzyme from the product

d)

It completely inhibits enzyme activity

8.

An immobilized enzyme shows a decrease in activity over multiple uses. What is the most practical first step to address this?

a)

Discard the enzyme beads immediately

b)

Add more substrate to compensate

c)

Analyze the beads for structural damage or enzyme leakage

d)

Change the enzyme to lipase

9.

What is the function of calcium chloride in the alginate immobilization process?

a)

To increase enzyme activity

b)

To stabilize the substrate

c)

To cross-link alginate and form gel beads

d)

To dissolve the enzyme

10.

After several cycles, you notice that immobilized amylase loses 20% of its initial activity. How would you apply your knowledge to improve bead performance?

a)

Reduce the temperature of reaction environment

b)

Increase the size of the amylase enzyme

c)

Add protease to the system

d)

Switch to a less porous alginate matrix or reinforce bead integrity