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Biology Concepts and Experimental Techniques

Total questions: 15

Worksheet time: 8mins

Name
Class
Date
1.

In a scientific experiment, what is the variable that is changed by the experimenter?

a)

Independent variable

b)

Dependent variable

c)

Control variable

d)

Extraneous variable

2.

What is a method to reduce parallax error when reading a measurement from a ruler or measuring cylinder?

a)

Taking multiple readings and calculating a mean

b)

Ensuring your eye is in line with the measurement

c)

Using a digital measuring device

d)

Repeating the experiment on different days

3.

When preparing an onion skin sample for microscopy, what is the purpose of adding a drop of iodine?

a)

To prevent the sample from drying out

b)

To increase the magnification of the cells

c)

To stain the cells for better visibility

d)

To preserve the cells from decay

4.

When starting to view a specimen under a microscope, which objective lens should be used first?

a)

The highest magnification objective lens

b)

The lowest magnification objective lens

c)

Any objective lens can be used first

d)

The objective lens with the largest field of view

5.

In an osmosis experiment with potato cylinders, what does the point where the line of best fit meets the x-axis on a graph of percentage change in mass vs. sugar concentration represent?

a)

The maximum water absorption by the potato cells

b)

The concentration of sugar in the potato cells

c)

The point where the potato cells burst

d)

The minimum water loss from the potato cells

6.

In an enzyme experiment involving starch, what color change indicates the continued presence of starch when iodine is added?

a)

Orange to black/dark purple

b)

Blue to green

c)

Yellow to orange

d)

Clear to cloudy

7.

Which reagent is used to test for the presence of protein in a food sample, and what color change indicates a positive result?

a)

Iodine solution, turning orange to black

b)

Benedict's solution, turning blue to brick red

c)

Biuret's reagent, turning blue to purple

d)

Cold ethanol, turning clear to cloudy

8.

What is the expected result when testing for lipids (fats) using cold ethanol followed by mixing with water?

a)

The solution turns black

b)

The solution turns green

c)

The solution turns purple

d)

The solution turns cloudy

9.

What is the independent variable when investigating the relationship between light intensity and the rate of photosynthesis?

a)

The volume of oxygen produced.

b)

The number of bubbles released.

c)

The distance between the plant and the light source.

d)

The amount of sodium hydrogen carbonate added.

10.

How does light intensity change if the distance from the light source is doubled?

a)

It doubles.

b)

It halves.

c)

It becomes one-quarter.

d)

It remains the same.

11.

Which of the following is a correct formula to calculate reaction time (t) based on the distance (s) a ruler drops, where 'a' is acceleration due to gravity?

a)

t = s / a

b)

t = 2s / a

c)

t = sqrt(2s / a)

d)

t = sqrt(s / 2a)

12.

What is the purpose of using a random number generator when placing quadrats to estimate an organism's population?

a)

To ensure all organisms are counted.

b)

To make the sampling process faster.

c)

To avoid bias in selecting sampling locations.

d)

To measure the exact area of the habitat.

13.

Why is it important to sterilize equipment using a flame when preparing bacteria cultures?

a)

To make the equipment easier to handle.

b)

To prevent contamination from airborne microbes.

c)

To increase the growth rate of bacteria.

d)

To make the agar solidify faster.

14.

What is the term for the growth of plant roots downwards in response to gravity?

a)

Phototropism

b)

Hydrotropism

c)

Geotropism

d)

Thigmotropism

15.

When investigating the decay of milk using lipase, what happens to the enzyme's activity if the temperature is too high?

a)

The enzyme's activity increases indefinitely.

b)

The enzyme denatures and its activity decreases.

c)

The enzyme becomes more stable.

d)

The enzyme changes color.