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Tracer Technique Quiz

Total questions: 10

Worksheet time: 8mins

Name
Class
Date
1.

Which detector uses low-pressure gas to detect ionizing radiation?

a)

NMR Spectrophotometer

b)

Gas Ionization Chamber

c)

GM Counter

d)

Autoradiography

2.

The labelled precursor is introduced into plants in the precursor-product sequence method to study:

a)

Metabolic disorders

b)

Rate of photosynthesis

c)

Biogenesis of plant constituents

d)

pH change in tissues

3.

Which technique gives information about bond cleavage and formation?

a)

Isotope incorporation

b)

Double labelling

c)

Competitive feeding

d)

Grafting

4.

In competitive feeding, which plant alkaloids are studied using ¹⁴C compounds?

a)

Tropane alkaloids

b)

Caffeine alkaloids

c)

Steroidal alkaloids

d)

Glycoalkaloids

5.

What is the primary use of the autoradiography technique?

a)

To determine isotope mass

b)

To study radiation emission

c)

To visualize radioactive material distribution

d)

To analyze protein folding

6.

Tritium is produced by:

a)

Gamma irradiation

b)

Neutron bombardment of lower hydrogen isotopes

c)

Electron transfer reactions

d)

DNA synthesis

7.

What must be considered while selecting a tracer compound?

a)

Must be yellow in color

b)

Must be non-reactive

c)

Must have high half-life and active participation in synthesis

d)

Should degrade quickly

8.

What kind of compound is used to study glucose in biological systems?

a)

¹⁵N-labelled nitrate

b)

¹⁴C-labelled glucose

c)

³²P-labelled ATP

d)

³⁵S-labelled protein

9.

What is the purpose of using double or multiple labelling techniques?

a)

To purify alkaloids

b)

To identify nature of precursor incorporation

c)

To destroy radioisotopes

d)

To measure chlorophyll content

10.

What is the significance of isotope incorporation technique?

a)

Identification of enzyme structure

b)

Understanding cleavage and bond formation in reactions

c)

Quantifying pigment concentration

d)

Increasing plant biomass