WorksheetsDNA Extraction Techniques in Biotechnology
Total questions: 8
Worksheet time: 8mins
Which of the following reagents is used to break open cell membranes during DNA extraction?
A) Isopropanol
B) Ethanol
C) Detergent
D) Salt
What is the purpose of adding salt during the DNA extraction process?
A) To denature proteins
charge on DNA
B) To neutralize the
C) To color the solution
D) To preserve RNA
True or False: Ethanol is used to break open the nuclear membrane?
A. True
B. False
Match the reagent with its function in DNA extraction:
Detergent
Breaks open cell membranes
Salt
Neutralizes DNA's charge
Protease
Breaks down proteins
Ethanol
Precipitates DNA
Which is a common troubleshooting step if no DNA is visible after extraction?
A) Increase centrifuge speed
B) Recheck ethanol concentration
C) Add more buffer
D) Skip incubation
Why might a biotechnology lab extract DNA from bacteria?
A) To generate energy
B) To modify RNA
C) To sequence recombinant plasmids
D) To grow colonies faster
Why might some protocols use isopropanol instead of ethanol?
A) It denatures DNA faster
B) It requires less volume to precipitate DNA
C) It breaks down proteins
D) It is more colorful
Reflect on a research scenario in biotechnology where you would extract DNA. How does understanding the process improve accuracy and outcomes in your lab?
