WorksheetsEnzymes & Functions (DNA REPLICATION)
Total questions: 7
Worksheet time: 4mins
Untwist the (parental) DNA double helix at the replication forks, separating the two parental DNA strands and making them available as template strands.
DNA polymerase I
DNA polymerase III
Topoisomerase
Helicase
Synthesized RNA primer by adding RNA nucleotides that is complementary to the DNA template from 5’ to 3’
DNA polymerase I
Primase
Topoisomerase
Helicase
Relieves overwinding strain (ahead of replication fork) by breaking, swiveling, and rejoining DNA strands.
DNA polymerase I
Primase
Topoisomerase
Helicase
Bind to the unpaired DNA strands, keeping them from re-pairing.
DNA polymerase III
Primase
Topoisomerase
Single-strand binding proteins
Remove RNA nucleotides of RNA primer from 5’ end and replace with DNA nucleotides added one by one (to the 3’ end of pre-existing DNA strand).
DNA polymerase I
Primase
DNA ligase
Single-strand binding proteins
Catalyze the synthesis of new DNA strand by adding DNA nucleotides to the 3′ end of RNA primer or pre-existing DNA strand using parental DNA strand as a template.
DNA polymerase I
DNA polymerase III
Helicase
Single-strand binding proteins
Catalyze the synthesis of phosphodiester bond to join the sugar-phosphate backbones of Okazaki fragments into a continuous DNA strand.
Topoisomerase
DNA ligase
Helicase
DNA polymerase I
