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Worksheets

4th session

Total questions: 72

Worksheet time: 36mins

Name
Class
Date
1.

The construction and use of the compound microscope is attributed to:

a)

Antonie van Leeuwenhoek

b)

Louis Pasteur

c)

Robert Koch

d)

Ferdinand Cohn

2.

The techniques of sterilization were introduced by:

a)

Louis Pasteur

b)

Robert Koch

c)

Ferdinand Cohn

d)

John Needham

3.

Bacillus of tuberculosis was discovered by:

a)

Hansen

b)

Loeffler

c)

Robert Koch

d)

Bruce

4.

The term microbiology, as the study of living organisms of microscopic size, was coined by:

a)

Antonie van Leeuwenhoek

b)

Robert Koch

c)

Louis Pasteur

d)

Edward Jenner

5.

The term vaccine was coined by:

a)

Edward Jenner

b)

Kitasato

c)

Ehrlich

d)

Louis Pasteur

6.

Rabies vaccine was developed for the first time in 1885 by:

a)

Louis Pasteur

b)

Semple

c)

Edward Jenner

d)

Paul Ehrlich

7.

Who discovered Mycobacterium tuberculosis and Vibrio cholerae?

a)

Loeffler

b)

Welch

c)

Pfeiffer

d)

Robert Koch

8.

Who introduced the method of vaccination to prevent smallpox?

a)

Louis Pasteur

b)

Edward Jenner

c)

Paul Ehrlich

d)

John Hunter

9.

Living organisms including bacteria and protozoa were first observed by:

a)

Louis Pasteur

b)

Robert Koch

c)

Antonie van Leeuwenhoek

d)

Christian Gram

10.

Who is known as father of antiseptic surgery?

a)

Alexander Fleming

b)

Joseph Lister

c)

Charles Nicolle

d)

Christian Gram

11.

Attenuated vaccine was first developed by:

a)

Louis Pasteur

b)

Robert Koch

c)

Landsteiner

d)

Ehrlich

12.

The event that triggered the development and establishment of microbiology as a science is the:

a)

Vaccinations

b)

Spontaneous generation

c)

Use of disinfectants

d)

Development of the microscope

13.

Which of the following is not a condition of Koch's postulates?

a)

Isolate the causative agent of a disease

b)

Cultivate the microbe in the laboratory

c)

Inoculate a test animal to observe the disease

d)

Produce a vaccine

14.

Peptidoglycan is major constituent of cell wall of:

a)

Gram-positive bacteria

b)

Gram-negative bacteria

c)

Fungi

d)

Protozoa

15.

Ultraviolet light is used in:

a)

Fluorescence microscope

b)

Dark ground microscope

c)

Phase contrast microscope

d)

Interference microscope

16.

Cell wall-deficient forms of Gram-positive bacteria are called:

a)

Spheroplasts

b)

Trophozoites

c)

Cysts

d)

Protoplasts

17.

Bacterial structure/s concerned with respiration is/are:

a)

Mesosomes

b)

Ribosomes

c)

Mitochondria

d)

Golgi apparatus

18.

Which of the following bacterial structures is/are involved in attachment to cell surface?

a)

Flagella

b)

Fimbriae

c)

Capsule

d)

Mesosomes

19.

Which of the following are eukaryotes?

a)

Fungi

b)

Bacteria

c)

Chlamydiae

d)

Mycoplasmas

20.

What function does a condenser serve in light microscope?

a)

Focuses the light onto our eyes.

b)

Focuses the light rays on the sample.

c)

Increases light intensity.

d)

Reduces glare.

21.

The best resolution that can be obtained with electron microscope is:

a)

0.1-0.2 nm

b)

0.3-0.5 nm

c)

0.6-0.7 nm

d)

0.8-0.9 nm

22.

Generation time of Escherichia coli is:

a)

20 seconds

b)

20 minutes

c)

14-15 hours

d)

12-13 days

23.

The period between inoculation of bacteria in a culture medium and beginning of multiplication is known as:

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Decline phase

24.

Bacteria whose optimum temperature for growth is 37ºC are known as:

a)

Mesophiles

b)

Psychrophiles

c)

Thermophiles

d)

Noctophiles

25.

All bacteria that inhabit the human body are:

a)

Autotrophs

b)

Heterotrophs

c)

Phototrophs

d)

Chemolithotrophs

26.

Chocolate agar is an example of which of the following?

a)

Basal media

b)

Selective media

c)

Differential media

d)

Enriched media

27.

Destruction or inhibition of microorganisms in living tissues is known as:

a)

Sterilization

b)

Disinfection

c)

Antisepsis

d)

None of the above

28.

Which of the following are referred to as 'jumping genes'?

a)

Plasmids

b)

Transposons

c)

Bacteriophages

d)

Operons

29.

The first vaccine for human use produced using DNA technology was:

a)

Polio vaccine

b)

MMR vaccine

c)

AIDS vaccine

d)

Hepatitis B vaccine

30.

Who is the father of genetics?

a)

Gregor Mendel

b)

Griffith

c)

Hargobind Khurana

d)

Jacob

31.

Which of the following infections is acquired from animals?

a)

Plague

b)

Diphtheria

c)

Meningococcal meningitis

d)

Poliomyelitis

32.

Which of the following infections is not spread by the respiratory route?

a)

Common cold

b)

Measles

c)

Mumps

d)

Hepatitis B

33.

Large number of bacteria forming normal flora are found:

a)

In the mouth

b)

On the skin

c)

In the large intestine

d)

In the nose

34.

Phagocytes are potent non-specific host defences. Which of the following are the major phagocytic cells in the body?

a)

B lymphocytes

b)

T lymphocytes

c)

Neutrophils

d)

Basophils

35.

Phylogenetically, the oldest immunoglobulin class is:

a)

IgA

b)

IgM

c)

IgG

d)

IgE

36.

B cells that produce and release large amounts of antibody are called:

a)

Memory cells

b)

Killer cells

c)

Basophils

d)

Plasma cells

37.

Most widely accepted theory of antibody production is:

a)

Direct template theory

b)

Indirect template theory

c)

Side chain theory

d)

Clonal selection theory

38.

A transplant between individuals of different animal species is known as:

a)

Xenograft

b)

Isograft

c)

Homograft

d)

Allograft

39.

The most important cause of bacterial sore throat is:

a)

Streptococcus pyogenes

b)

Staphylococcus aureus

c)

Haemophilus spp.

d)

Mycoplasma pneumoniae

40.

Which of the following is optimal specimen to be collected for the diagnosis of gonorrhoea by culture in males?

a)

Urine

b)

Urethral swab

c)

Rectal swab

d)

Pharyngeal swab

41.

Which of the following bacteria are predominant flora of the human gut?

a)

Escherichia coli

b)

Anaerobic bacteria

c)

Enterococcus faecalis

d)

Actinomyces

42.

Which of the following is the most common zoonotic infection in the world?

a)

Rat bite fever

b)

Anthrax

c)

Leptospirosis

d)

Plague

43.

Plague or the black death is transmitted by:

a)

Sandflies

b)

Rat fleas

c)

Mites

d)

Ticks

44.

Which of the following is RNA virus?

a)

Papilloma virus

b)

Influenza virus

c)

Hepatitis B virus

d)

Epstein-Barr virus

45.

Which of the following is DNA virus?

a)

Polio virus

b)

Rubella virus

c)

Vaccinia virus

d)

Human immunodeficiency virus

46.

A chemical component that is found in all viruses is

a)

Protein

b)

RNA

c)

DNA

d)

Lipid

47.

Viruses range in size from:

a)

1 to 100 nm

b)

10 to 300 nm

c)

300 to 500 nm

d)

500 to 750 nm

48.

Which is the commonest mode of transmission of HIV?

a)

Sexual

b)

Parenteral

c)

Perinatal

d)

Oral

49.

Following are the examples of re-emerging infections, except:

a)

Malaria

b)

Plague

c)

AIDS

d)

Typhoid fever

50.

Genital discharge is present in:

a)

Chancroid

b)

Gonorrhoea

c)

Herpes genitalis

d)

Lymphogranuloma venereum

51.

Unicellular parasites belong to kingdom:

a)

Monera

b)

Protozoa

c)

Plantae

d)

Animalia

52.

Helminths belong to kingdom:

a)

Monera

b)

Protozoa

c)

Plantae

d)

Animalia

53.

A parasite that must spend at least part of its life cycle on or in a host is called:

a)

Facultative parasite

b)

Hyperparasite

c)

Obligate parasite

d)

Pathogenic parasite

54.

Phylum Sporozoa contains

a)

Malaria parasites

b)

Ciliates

c)

Flagellates

d)

Amoebae

55.

Most common organ involved in extraintestinal amoebiasis is:

a)

Liver

b)

Lung

c)

Brain

d)

Spleen

56.

Reservoir host of Entamoeba histolytica is:

a)

Man

b)

Pig

c)

Dog

d)

Sheep

57.

Who first found and described malaria parasite?

a)

Charles Laveran

b)

Ronald Ross

c)

Robert Koch

d)

Carlos Chagas

58.

The parasitic helminths that are most likely to be found in bile ducts, lungs and blood are the:

a)

Tapeworms

b)

Roundworms

c)

Flatworms

d)

Flukes

59.

Which of the following is true of the microbial world?

a)

All microbes are harmful.

b)

Humans could survive without microbes.

c)

Many microbes are helpful

d)

Most microbes are pathogenic

60.

To reduce the risk of open flame from Bunsen burners, microbiologists are increasingly using which of the following devices for easy, rapid benchtop sterilization of small pieces of equipment?

a)

autoclaves

b)

ethylene oxide

c)

incubators

d)

microincinerators

61.

Ernst Haeckel proposed a classification system consisting of which of the following subgroups?

a)

five kingdoms

b)

Three kingdoms

c)

two domains and three kingdoms

d)

two domains and five kingdoms

62.

Viruses not included in phylogenetic trees for which of the following reasons?

a)

It is too difficult to observe their characteristics.

b)

They are acellular and nonliving.

c)

They do not have any genes, which prevents genetic analysis.

d)

They lack a nucleus.

63.

For taxonomic classification of microbes, serological tests typically are used to do which of the following?

a)

determine complete genome sequences for microbes

b)

identify proteins on the basis of their reaction with specific antibodies

c)

run analyses to identify whether particular genes are present

d)

visualize microbial external morphology

64.

Which of the following indicates how bacteria and viruses typically compare in size?

a)

Bacteria are indistinguishable from viruses.

b)

Bacteria are much larger than viruses

c)

Bacteria are much smaller than viruses.

d)

Bacteria are similar in size to viruses.

65.

Chitin cell walls are characteristic of which taxonomic group?

a)

algae

b)

archaea

c)

bacteria

d)

fungi

66.

The cell walls of archaeans often contain which of the following substances?

a)

cellulose

b)

chitin

c)

peptidoglycan

d)

pseudopeptidoglycan

67.

Mycotoxins are produced by which type of organism?

a)

archaeans

b)

heterotrophic protists

c)

Molds

d)

protozoans

68.

Which of the following is true of the genetics of viruses?

a)

Viruses always contain DNA and force host cells to produce RNA.

b)

Viruses always contain DNA and RNA.

c)

Viruses always contain RNA and force host cells to produce DNA.

d)

Viruses can contain either DNA or RNA, but not both.

69.

Which microorganisms are used to make microbiological growth media?

a)

bacteria

b)

fungi

c)

algae

d)

protozoa

70.

Of the following scientists, who first promulgated the theory of abiogenesis?

a)

Aristotle

b)

Pasteur

c)

Needham

d)

Spallanzani

71.

A nanometer is______than a micrometer

a)

10 times larger

b)

10 times smaller

c)

1000 times larger

d)

1000 times smaller

72.

Which recombinant DNA technique is used to replicate copies of a DNA molecule?

a)

PCR

b)

gel electrophoresis

c)

electroporation

d)

reverse transcription