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WorksheetsHPLC Quiz :Round 1 Qualifiers
Total questions: 20
Worksheet time: 7mins
Additive to regulate pH in HPLC ?
Acetonitrile
Buffer
Methanol
Purge
U-HPLC differs from conventional HPLC mainly because of
Higher column temperatures
Different detectors
Mobile phase recycling
Smaller particle size columns
Which compound will elute faster in reverse-phase HPLC
highly polar
Non polar
Hydrophobic
Unionisable
The effective buffering range of a weak acid buffer is approximately:
pKa ± 0.5
pKa ± 1
pKa ± 2
pKa ± 3
If the calibration curve consistently shows lower response at higher concentrations, the likely cause is
At pH > 8, what happens to silica?
They get protonated
They get deprotonated
Ligand gets detached
Silica will dissolve
Drastic shift in retention time of drug peak indicates
Incorrect flow rate or pump malfunction
Detector lamp instability
Altered pH of buffer
Increase in column pressure
During analysis, all peaks show severe tailing, especially for basic drugs The most likely cause is:
Loss of bonded phase
Interaction with residual silanols
Causing Column void formation
Detector misalignment
Column storing solvent?
0.1% formic acid
Buffer
Methanol
Diluted KCl
Autosampler injection volume unit?
Microliter (µL)
Millilitre (Ml)
Litre (L)
Nanoliter(nL)
If you dissolve 2mg of analyte in 2ml of solvent , then parent stock solution concentration should be
1 ug/uL
1 ng/uL
1 g/dL
1ug/ml
Main principle of Solid Phase Extraction is
Adsorption
Resorption
Partioning of analytes between 2 layers
A and B
Find out the 2of32s westgard multirule from the following
when 3 consecutive Qc measurements exceed the same mean plus 1s or mean minus 1s control limit
when 2 out of 3 Qc measurements exceed the same mean plus 2s or mean minus 2s control limit
when 3 consecutive Qc measurements exceed the same mean plus 1s or mean minus 1s control limit
None of the above
High backpressure in HPLC may be due to
Blocked frit in column
Mobile phase degassed
Detector lamp intensity low
Increase in column temperature
Identify the given instrument
Filtration Apparatus
Solid phase extraction
Vaccum Fume hood
Vacuum manifold
In Liquid-Liquid Extraction, a weakly basic drug (pKa - 8) is best extracted from plasma at which pH?
Strongly acidic pH (<3)
Strongly basic pH (>10)
Neutral pH (7)
No effect of pH
pH of mobile phase is 9, then which column will prefer for HPLC analysis
C18 column
C8 column
Hilic column
Bridged Hybrid column
All are polar solvent EXCEPT
Acetonitrile
Hexane
Ammonium acetate
Zinc Sulphate
From the display, note the current pressure of HPLC system
2677
3000
254
1500
