Worksheetshtmle-draft10
Total questions: 116
Worksheet time: 58mins
Name
Class
Date
1.
classified into Simple Lipids, Compound lipids and Derived Lipids
a)
Staining for Lipids
b)
Staining of Proteins, Enzymes and Nucleic Acids
c)
Staining of Connective Tissues
2.
- Best demonstrated on Frozen Sections. Fresh, unfixed tissues.
a)
Staining for Lipids
b)
Staining of Proteins, Enzymes and Nucleic Acids
c)
Staining of Connective Tissues
3.
Phospholipids and Neutral Fats are lost during routine dehydration and embedding (unless treated with Potassium Dichromate or Osmic acid
a)
Staining for Lipids
b)
Staining of Proteins, Enzymes and Nucleic Acids
c)
Staining of Connective Tissues
4.
the fixative of Choice for Lipid Histochemistry.
a)
Formol Calcium
b)
Sudanophilia
5.
- suitable for cryostat sections
a)
Formol Calcium
b)
Sudanophilia
6.
- mounted on chrome-gelatin coated slides.
a)
Formol Calcium
b)
Sudanophilia
7.
Formol Calcium is a combination of..
a)
10% Formalin
b)
2% Formalin
c)
4% Formalin
d)
10% Calcium Acetate
e)
2% Sodium Acetate
8.
Which type of lipid involve Triglycerides which are stainable with Sudan B, Sudan IV and Oil Red O
a)
Simple lipids
b)
Compound lipids
c)
Derived Lipids
9.
Which type of lipid is Consisted of Fatty acid, alcohol or one or more other groups such as phosphorus or nitrogen? This is Found in Central Nervous System
a)
Simple lipids
b)
Compound lipids
c)
Derived Lipids
10.
Which type of lipid involve Fatty acids from hydrolysis of simple and compound lipids?
a)
Simple lipids
b)
Compound lipids
c)
Derived Lipids
11.
Examples of this type of lipids are cholesterol, Bile acids, Sex Hormones and adrenocortical hormones.
a)
Simple lipids
b)
Compound lipids
c)
Derived Lipids
12.
found in Mitochondria and Nervous tissue elements
a)
Phospholipids
b)
Glycolipids
13.
consisted of Fatty acids and Hexoses
a)
Phospholipids
b)
Glycolipids
14.
Stain for Phospholipids
a)
Sudan Black B
b)
Acid Hematin
c)
PAS
15.
Stain for Glycolipids
a)
Sudan Black B
b)
Acid Hematin
c)
PAS
16.
– Original diazo dye
a)
Basic aryl amines
b)
B-napthols
17.
Sudan Black B is a type of.. oil soluble dyes under
a)
Basic aryl amines
b)
B-napthols
18.
Sudan III is a type of oil soluble dyes under...
a)
Basic aryl amines
b)
B-napthols
19.
– Most sensitive lipid stain and Sudan Red VII B
a)
Sudan Black B
b)
Sudan IV
c)
Sudan III
20.
( C.I No. 26100) (1896)
a)
Sudan Black B
b)
Sudan IV
c)
Sudan III
21.
(Scharlach B) (1901)
a)
Sudan Black B
b)
Sudan IV
c)
Sudan III
22.
more brilliant or Deeper red color compared to Sudan III
a)
Sudan Black B
b)
Sudan IV
c)
Sudan III
23.
– very low water solubility
a)
Basic aryl amines
b)
B-napthols
24.
Introduced in 1926; Popularized by Lillie and Ashburn in 1943; Stains Neutral Fats and Lipofuscin
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
25.
Uses 70% Alcohol for solvent for Oil Red O and Sudan Black.
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
26.
Fats stain Brilliant Red
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
27.
Not a dye but is reduced by unsaturated fats and fatty acids into black.
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
28.
Nuclei is yellow-orange
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
29.
Two components
Red Oxazone – dissolves Neutral Lipids
Blue Oxazine – basic component and interacts with Phospholipid and Free Fatty Acid
a)
Oil Red O
b)
Oil Red O in Dextrin
c)
Osmic Acid
d)
Nile Blue Sulfate
30.
Which component of nile blue sulfate dissolves neutral lipids?
a)
Red Oxazone
b)
Blue Oxazine
31.
Which component of nile blue sulfate is the basic component that interacts with Phospholipid and free fatty acid?
a)
Red Oxazone
b)
Blue Oxazine
32.
Stains pinkish red in Nile Blue Sulfate
a)
Neutral fats
b)
Cholesterin Esters and Fatty acids
c)
Cerebrosides
d)
Fatty acid and Soap
33.
Stains light red in Nile Blue Sulfate
a)
Neutral fats
b)
Cholesterin Esters and Fatty acids
c)
Cerebrosides
d)
Fatty acid and Soap
34.
Stains light blue in Nile Blue Sulfate
a)
Neutral fats
b)
Cholesterin Esters and Fatty acids
c)
Cerebrosides
d)
Fatty acid and Soap
35.
Stains deep blue to violet in Nile Blue Sulfate
a)
Neutral fats
b)
Cholesterin Esters and Fatty acids
c)
Cerebrosides
d)
Fatty acid and Soap
36.
Neutral fats in Nile Blue Sulfate
a)
Pinkish red
b)
Light Red
c)
Light blue
d)
Deep blue to violet
37.
Cerebrosides in Nile Blue Sulfate
a)
Pinkish red
b)
Light Red
c)
Light blue
d)
Deep blue to violet
38.
Fatty acid and soap in Nile Blue Sulfate
a)
Pinkish red
b)
Light Red
c)
Light blue
d)
Deep blue to violet
39.
Cholesterin Esters and Fatty acids in Nile Blue Sulfate
a)
Pinkish red
b)
Light Red
c)
Light blue
d)
Deep blue to violet
40.
binds heavy metal ions like copper to form soaps (Stained by Weigert’s Lithium Hematoxylin – Fischler 1904)
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
41.
Dimethylaminobenzylidine Rhodanine (Okamoto et. Al 1944) or Rubeanic Acid (Holczinger 1959)
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
42.
Must be oxidized first before staining;
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
43.
Enzymatic Method by Emeis et. Al in 1977 (Use of Cholesterol esterase and Cholesterol Oxidase)
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
44.
Use of Periodic Acid Schiff to stain Mucopolysaccharides, can be distinguished from glycogen by removal with diastase
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
45.
For Patients suffering from Sulfatide Storage disease known as Metachromatic Leukodystrophy (Staining by Cresyl Violet)
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
46.
- Producing Metachromatic Orange color on Sulfatides
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
47.
TOLUIDINE BLUE – ACETONE METHOD (Bodian and Lake 1963) – for Sulfatide: Metachromatic Red-Brown or Yellow
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
48.
Seen in Tay-Sach’s Disease and Gm1 Gangliosidosis – stained by conventional PAS methods
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
49.
BOROHYDRIDE – PERIODIC – SCHIFF (BHPS) METHOD – for Gangliosides: stained Red
a)
Free Fatty Acids
b)
Cholesterol
c)
Cerebroside
d)
Gangsliosides
50.
– selective staining with small or large molecule dyes (Trichrome method) and Silver Impregnation (Reticulin Method)
a)
Fibrous proteins
b)
Specific Dye-Protein Interactions
c)
Neutral Buffered Formol Saline
51.
Congo Red for Amyloid
a)
Fibrous proteins
b)
Specific Dye-Protein Interactions
c)
Neutral Buffered Formol Saline
52.
Most commonly used fixative in Amino Acid Histochemistry.
a)
Fibrous proteins
b)
Specific Dye-Protein Interactions
c)
Neutral Buffered Formol Saline
53.
Which Protein technique is For Protamines qnd Histones in the cell nuclei.. --
a)
Alkaline Fast Green Method
b)
Peracetic Acid - Alcian Blue
54.
Which Protein technique is For Cystine and Cysteine
a)
Alkaline Fast Green Method
b)
Peracetic Acid - Alcian Blue
55.
Peracetic Acid oxidizes these two amino acids to form strong cysteic acid which stained blue-green by a basic dye
a)
Alkaline Fast Green Method
b)
Peracetic Acid - Alcian Blue
56.
Sakaguchi’s Test – For Arginine used Sodium Hydroxide, Sodium Hypochlorite or Milton’s reagent and Pyridine Chloroform. (Stains Orange-Red Color)
a)
Alkaline Fast Green Method
b)
Peracetic Acid - Alcian Blue
57.
Alkaline Phosphatase activity – Brownish Black
a)
Gomori Calcium Method
b)
Gomori Lead Method
c)
Lead Method (Wachstein and Meisel 1957)
d)
ATPase metal precipitation
58.
Acid Phosphatase activity – Black
a)
Gomori Calcium Method
b)
Gomori Lead Method
c)
Lead Method (Wachstein and Meisel 1957)
d)
ATPase metal precipitation
59.
5’ Nucleotidase – Blackish – Brown Deposits
a)
Gomori Calcium Method
b)
Gomori Lead Method
c)
Lead Method (Wachstein and Meisel 1957)
d)
ATPase metal precipitation
60.
Done on Acidic (4.2 to 4.6) and Basic Ph (9.4)
a)
Gomori Calcium Method
b)
Gomori Lead Method
c)
Lead Method (Wachstein and Meisel 1957)
d)
ATPase metal precipitation
61.
Non-specific Esterase – Reddish Brown
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
62.
Non-specific Esterase – Blue
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
63.
Colored Dark Brown to Black
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
64.
For Monoamine Oxidase (colored Bluish Black)
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
65.
Breakdown of Adrenaline and 5-hydroxytryptamine
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
66.
Oxidation of the substrate Tetranitro-blue tetrazolium (TNBT)
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
67.
Black or Bluish Black
a)
Alpha-naphthyl acetate
b)
Indoxyl acetate (Holts and Withers 1952)
c)
Acethylcholinesterase (Filipe and Lake 1983)
d)
Tetrazolium method (Glenner et. Al 1957)
e)
Succinic Dehydrogenase Enzyme
68.
Red purple
a)
Feulgen technique for nuclear DNA
b)
Methyl green - pyronin emthod for RNA and DNA
c)
Fluorescent staining for DNA and RNA
69.
DNA (Chromatin) is colored Green or Blue Green; RNA (Nucleoli) is colored Rose red; granules are dark rose-red
a)
Feulgen technique for nuclear DNA
b)
Methyl green - pyronin emthod for RNA and DNA
c)
Fluorescent staining for DNA and RNA
70.
FLUORESCEIN – widely used, wide absorption spectrum and blue light range
*Apple green emission in Mammalian tissue
RHODAMINE – Orange red emission ACRIDINE ORANGE
– most commonly used in DNA and RNA ACRIFLAVINE
– alternative to basic fuchsin in Schiff’s reagent
a)
Feulgen technique for nuclear DNA
b)
Methyl green - pyronin emthod for RNA and DNA
c)
Fluorescent staining for DNA and RNA
71.
detection of abnormal genes, viral infections and for tumor phenotyping
a)
In Situ hybridization
b)
Polymerase chain reaction
72.
– provides the means of detecting single copies of nucleic acid sequences in cellular preparations.
a)
In Situ hybridization
b)
Polymerase chain reaction
73.
Color of Reticulin in Gomoro's Silver Impregnation
a)
Black
b)
Pink to deep red
c)
Dark blue or Blue Black
74.
Color of Collagen in Van Gieson Stain
a)
Black
b)
Pink to deep red
c)
Dark blue or Blue Black
75.
Color of Elastic fiber in Weigert's stain
a)
Black
b)
Pink to deep red
c)
Dark blue or Blue Black
76.
Color of Elastic fiber in Verhoeff's elastic
a)
Black
b)
Pink to deep red
c)
Dark blue or Blue Black
77.
Reticulin – Black
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
78.
Reticulin – Black
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
79.
Collagen – Pink to Deep Red
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
80.
- Muscle, RBC and Keratin – Red Collagen and Mucus - Blue
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
81.
SOLUTION A – Acid Fuchsin, Glacial Acetic Acid and Distilled Water
SOLUTION B – Phosphomolybdic Acid and Distilled Water SOLUTION
C – Methyl Blue, Glacial Acetic Acid and Distilled Water
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
82.
Collagen fibers and Reticulum
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
83.
*Also stains Hyaline fibrils, fibroglia fibrils, smooth and striated muscle fibers and amyloid
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
84.
Heidenhain’s Modification of Mallory’s Aniline Blue staining. Uses Azocarmine as a counterstain.
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
85.
More useful in Basement membrane
a)
Gomoro's SIlver Impregnation
b)
Van GIeson's stain
c)
Mallory's aniline blue
d)
Azocarmine stain
e)
Trichrome stain
86.
Elastic Fiber – Dark Blue or Blue Black
a)
Weigert's stain
b)
Verhoeff's elastic
c)
Orcein (Taenzer - Unna Orcein)
d)
Krajian's
87.
Elastic Fiber – Black
a)
Weigert's stain
b)
Verhoeff's elastic
c)
Orcein (Taenzer - Unna Orcein)
d)
Krajian's
88.
Used in Dermatology
a)
Weigert's stain
b)
Verhoeff's elastic
c)
Orcein (Taenzer - Unna Orcein)
d)
Krajian's
89.
Rapid method for elastic fibers, fibrin and amyloid employing Congo Red
a)
Weigert's stain
b)
Verhoeff's elastic
c)
Orcein (Taenzer - Unna Orcein)
d)
Krajian's
90.
Acid Fuchsin, Glacial Acetic Acid and Distilled Water
a)
Trichrome Solution A
b)
Trichrome SOlution B
c)
Trichrome Solution C
91.
Phosphomolybdic Acid and Distilled Water
a)
Trichrome Solution A
b)
Trichrome SOlution B
c)
Trichrome Solution C
92.
Methyl Blue, Glacial Acetic Acid and Distilled Water
a)
Trichrome Solution A
b)
Trichrome SOlution B
c)
Trichrome Solution C
93.
Muscle, RBC and Keratin in Trichrome
a)
Red
b)
Blue
c)
Black
d)
Dark blue or Blue Black
94.
Collagen and Mucus in Trichrome
a)
Red
b)
Blue
c)
Black
d)
Dark blue or Blue Black
95.
MSB Technique (Lendrum et.al 1962) stains Red
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
96.
MALLORY’S PHOSPHOTUNGSTIC ACID HEMATOXYLIN
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
97.
Eosinophilic material; identical staining reaction with Fibrin
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
98.
Mixture of Exudate and Altered Cytoplasmic Constituents
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
99.
*Found in SLE, Collagen Disorders, Hypersensitivity, Rheumatic Heart Disease
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
100.
*Found in Necrotizing Vasculitis
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
101.
Wide variety of pathologic exudates and deposits
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
102.
Appears Smooth homogenous eosinophilic material in routine stains (in Degenerated Collagen,
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
103.
Hypertension, Atheroma and Diabetic Kidney)
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
104.
Non-specifically stained by PAS
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
105.
Semi-translucent; ground glass, or hyaline eosinophilic substance
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
106.
Made up of Chondroitin sulfuric acid-protein complexes (In CT, kidneys, splee, adrenals, lymph nodes, pancreas and during suppurative and inflammatory conditions like TB, Leprosy and Osteomyelitis
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
107.
GRAM’S IODINE – delicate purple to blue color
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
108.
GRAM’S IODINE – delicate purple to blue color
a)
Fibrin
b)
Fibrinoid
c)
Hyalin
d)
Amyloid
e)
Induced fluorescent staining
109.
delicate purple to blue color
a)
Congo Red
b)
Gram's iodine
c)
Highman's Congo Red 1946
d)
Alkaline Congo red (Puchtler et. al 1962)
e)
Krajian's modified benhold method
110.
– Deep pink to red color with subsequent birefringence under polarizing lens (Sine Qua non for Amyloid)
a)
Congo Red
b)
Gram's iodine
c)
Highman's Congo Red 1946
d)
Alkaline Congo red (Puchtler et. al 1962)
e)
Krajian's modified benhold method
111.
– Deep Pink to Red
a)
Gram's iodine
b)
Highman's Congo Red 1946
c)
Alkaline Congo red (Puchtler et. al 1962)
d)
Krajian's modified benhold method
e)
High pH Congo Red (Eastwood and COle 1971)
112.
Red
a)
Gram's iodine
b)
Highman's Congo Red 1946
c)
Alkaline Congo red (Puchtler et. al 1962)
d)
Krajian's modified benhold method
e)
High pH Congo Red (Eastwood and COle 1971)
113.
use of Harris Hematoxylin and Congo Red
a)
Gram's iodine
b)
Highman's Congo Red 1946
c)
Alkaline Congo red (Puchtler et. al 1962)
d)
Krajian's modified benhold method
e)
High pH Congo Red (Eastwood and COle 1971)
114.
*Amyloid – Yellow Fluorescence
a)
Thioflavine T
b)
Use of UV light source (Mercury vapor Lamps), UGI Exciter Filters, BG38 Red Suppresor Filter andK430 Barrier
c)
Use of Blue Light Fluorescence Quartz-Iodine or Mercury Vapor Lamp with BG12 Exciter filter and K530 Filte
115.
For staining Amyloid and other substance
a)
Thioflavine T
b)
Use of UV light source (Mercury vapor Lamps), UGI Exciter Filters, BG38 Red Suppresor Filter andK430 Barrier
c)
Use of Blue Light Fluorescence Quartz-Iodine or Mercury Vapor Lamp with BG12 Exciter filter and K530 Filte
116.
*Amyloid – Silver Blue Fluorescence
a)
Thioflavine T
b)
Use of UV light source (Mercury vapor Lamps), UGI Exciter Filters, BG38 Red Suppresor Filter andK430 Barrier
c)
Use of Blue Light Fluorescence Quartz-Iodine or Mercury Vapor Lamp with BG12 Exciter filter and K530 Filte
100 %
