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WorksheetsDNA Extraction and Analysis Quiz
Total questions: 10
Worksheet time: 5mins
Which of the following bases are classified as purines?
Adenine and Cytosine
Guanine and Thymine
Adenine and Guanine
Cytosine and Thymine
In DNA extraction, the purpose of using isopropanol or ethanol is to:
Break down proteins
Precipitate DNA
Dissolve lipids
Separate RNA from DNA
During phenol/chloroform extraction, nucleic acids are found in which layer after centrifugation?
Organic (lower) layer
Aqueous (upper) layer
Interface layer
Bound to phenol phase
Which of the following differentiates DNA from RNA?
DNA contains ribose sugar while RNA contains deoxyribose
DNA is single-stranded while RNA is double-stranded
DNA contains thymine while RNA contains uracil
Both contain uracil as a nitrogenous base
A researcher extracts DNA from banana using detergent, salt, and alcohol. The detergent function in this step is:
To precipitate DNA
To denature proteins
To dissolve cell membranes by breaking lipids
To stabilize DNA structure
A student observes smeared DNA bands on agarose gel electrophoresis after extraction from blood. The most likely cause is:
Contamination with RNA
Overloading of the gel with DNA
Incomplete protein removal
All of the above
Proteinase K is added during DNA extraction to:
Digest polysaccharides
Break down proteins and nucleases
Precipitate DNA
Enhance lipid dissolution
A farmer in Egypt uses Bt cotton to reduce pesticide use. The gene responsible for pest resistance is inserted using:
Lipid nanoparticles
Agrobacterium-mediated transformation
CRISPR-Cas9 editing
Electroporation
A patient’s tumor DNA is being sequenced to identify mutations for personalized therapy. The primary reason for DNA purification before sequencing is:
To remove water
To eliminate proteins and other contaminants that interfere with enzymes
To increase DNA double helix stability
To convert DNA to RNA for easier analysis
In the development of COVID-19 vaccines, synthetic mRNA was produced from DNA templates. This demonstrates which principle of biotechnology?
Reverse transcription from RNA to DNA
Use of recombinant DNA to generate coding sequences
Gene silencing using siRNA
DNA replication by DNA polymerase III
