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[LRH] Bacteriology Primer Part 1

Total questions: 93

Worksheet time: 1hrs 3mins

Name
Class
Date
1.

Their DNA is not enclosed within a membrane 

a)

PROKARYOTES

b)

EUKARYOTES

2.

Their DNA is not associated with histones

a)

PROKARYOTES

b)

EUKARYOTES

3.

PROKARYOTES THAT DOES NOT HAVE CELL WALL

(a)  

4.

Prokaryotes DNA is enclosed in (a)  

5.

Prokaryotes divide through

a)

Mitosis

b)

Meiosis

c)

Binary Fusion

d)

Binary Fission

6.

Their DNA is found in the cell’s nucleus

a)

PROKARYOTES

b)

EUKARYOTES

7.

Eukaryotes diviide through

a)

Mitosis

b)

Meiosis

c)

Binary Fusion

d)

Binary Fission

8.

VIRUSES ARE CONSIDERED (select multiple)

a)

CELLULAR

b)

ACELLULAR

c)

HAS A CELL WALL

d)

INFECTIOUS AGENTS

9.

True about Eubacteria

a)

Blue green algae

b)

Survives in extreme environment

c)

True bacteria

10.

True about Cyanobacteria

a)

Blue green algae

b)

Survives in extreme environment

c)

True bacteria

11.

True about Archaebacteria

a)

Blue green algae

b)

Survives in extreme environment

c)

True bacteria

12.

True about Prokaryotes

a)

has a cell membrane

b)

DNA is enclosed in a nucleus

c)

Divides by mitosis

d)

They exhibit nucleoid.

13.

True about Eukaryotes

a)

They divide by binary fission

b)

Animals have no cell wall

c)

Has cell membrane

d)

Phospholipid bilayer does not contain sterol 

14.

Yeasts are…

a)

Single-cell prokaryotic microorganisms

b)

Single-cell or multicellular eukaryotic organisms

c)

Unicellular, eukaryotic organisms

d)

Dependent on host cells for survival and therefore are not considered cellular organisms but rather INFECTIOUS AGENTS

15.

Fungi are..

a)

Single-cell prokaryotic microorganisms

b)

Single-cell or multicellular eukaryotic organisms

c)

Unicellular, eukaryotic organisms

d)

Dependent on host cells for survival and therefore are not considered cellular organisms but rather INFECTIOUS AGENTS

16.

Viruses are..

a)

Single-cell prokaryotic microorganisms

b)

Single-cell or multicellular eukaryotic organisms

c)

Unicellular, eukaryotic organisms

d)

Dependent on host cells for survival and therefore are not considered cellular organisms but rather INFECTIOUS AGENTS

17.

Bacteria are…

a)

Single-cell prokaryotic microorganisms

b)

Single-cell or multicellular eukaryotic organisms

c)

Unicellular, eukaryotic organisms

d)

Dependent on host cells for survival and therefore are not considered cellular organisms but rather INFECTIOUS AGENTS

18.

Parasites are…

a)

Single-cell or multicellular eukaryotic organisms

b)

Unicellular, eukaryotic organisms

c)

Dependent on host cells for survival and therefore are not considered cellular organisms but rather INFECTIOUS AGENTS

19.

Site of energy production for prokaryotes

a)

Mitochondria

b)

Free Ribosomes

c)

Rough ER

d)

Cytoplasmic Membrane

20.

Cell envelope of a bacteria is composed of

a)

Outer Membrane for gram negative

b)

Cell wall composed of peptidoglycan

c)

Periplasm for gram negative bacteria

d)

cytoplasmic or cell membrane

e)

All of the above

21.

Defines the shape of the bacteria

(a)  

22.

Peptidolycan layer composed of alternating N-ACETYL-D-GLUCOSAMINE

(NAG) AND N-ACETYL-D-MURAMIC ACID (NAM)

a)

False

b)

True

23.

Murein Layer is composed of

a)

N-ACETYL-D-GLUCOSAMINE

(NAG) AND N-ACETYL-D-MURAMIC ACID (NAM)

b)

N-GLYCOLYLMURAMIC ACID

24.

Mycobacterium cell wall structure is composed of

a)

N-ACETYL-D-GLUCOSAMINE

(NAG) AND N-ACETYL-D-MURAMIC ACID (NAM)

b)

N-GLYCOLYLMURAMIC ACID

25.

Identify the correct order

a)

Primary Stain - Decolorizer -Mordant - Counterstain

b)

Decolorizer - Primary Stain - Counterstain - Mordant

c)

Crystal Violet - Grams Iodine - Alcohol - Safranin

d)

Safranin - Grams Iodine - Alcohol - Crystal Violet

26.

Gram Negative bacteria after decolorizing

a)

Pink

b)

Red

c)

Colorless

d)

Violet

27.

What is the purpose of the mordant?

a)

to complete the process as it is the last step

b)

for decolorizer to function

c)

link dyes to tissue

d)

its not useful and can be removed when urgent

28.

Virulence factor that inhibits phagocytosis and is found in the cell wall

(a)  

29.

The bacterial cell wall is composed of 70% water and 30% carbohydrates, proteins, lipids etc

a)

True

b)

False

30.

True about gram positive

a)

does not stain at all after gram staining

b)

has thick peptidoglycan and has techoic acid that binds to magnesium

c)

has perisplasmic space

d)

cell wall contains lipopolysaccharide

31.

Examples of spirochetes

a)

Treponema

b)

Balantidium

c)

Leptospira

d)

Borrelia

32.

What stain shall be used for Legionella?

a)

Crystal Violet

b)

Methylene Blue

c)

Basic Fuchsin

d)

Carbolfuchsin

33.

Select the Gram positive cocci aerobes

a)

Staphylococcus, Micrococcus, Streptococcus, Neisseria

b)

Staphylococcus, Micrococcus, Neisseria

c)

Micrococcus, Streptococcus, Neisseria

d)

Micrococcus, Staphylococcus, Streptococcus

34.

Select the Catalase Positive organism

a)

staphylococcus

b)

microccocus

c)

streptococcus

35.

gram positive cocci, catalase negative

(a)  

36.

All cocci are gram positive except

a)

Neisseria

b)

Staphylococcus

c)

Moraxella

d)

Veillonella

37.

gram positive bacilli and h2s positive

(a)  

38.

erysipelothrix characteristics

a)

h2s variable

b)

cocci

c)

bacilli

d)

gram positive

e)

h2s positive

39.

What are the fungus like bacteria

a)

nocardia

b)

neisseria

c)

listeria

d)

actinomyces

40.

true about acid fast staining

a)

stains the lipopolysaccharide of the cell wall

b)

uses carbolfuchsin as the counterstain

c)

malachite green can be used as counterstain

d)

the size of the specimen must be 2x3cm or thumb sized

41.

what is the color of non acid fast organism

(a)  

42.

what isbthe color of gram positive organism

(a)  

43.

TRUE OR FALSE: KINYOUN METHOD IS NOT PREFERRED

a)

False

b)

True

44.

Mycolic Acid Cell wall except

a)

Nocardiopsis

b)

Nocardia

c)

Gordonia

d)

Corynebacterium

45.

Mycolic Acid Cell wall except

a)

Tsukamurella

b)

Dermatophilus

c)

Gordonia

d)

Corynebacterium

46.

site of energy production for prokaryotes

(a)  

47.

point of attachment for chromosomes

(a)  

48.

Inclusion of Mycobacterium Tuberculosis

(a)  

49.

Inclusion of C. diphtheria

(a)  

50.

select all inclusions of C. diphtheriae

a)

much granules

b)

volutin granules

c)

metachromatic granules

d)

babes ernst

51.

inclusion body of y. pestis

(a)  

52.

True about endospore

a)

made up of dipicolinic acid

b)

a toxin

c)

resting cell

d)

accountsnfor resistance in dessication, heat, and chemicals

53.

specie that is gram positive, aerobic, catalase positive

(a)  

54.

gram positive bacilli, anaerobe, catalase negative

(a)  

55.

virulence by preventing phagocytosis

(a)  

56.

also known a pili

(a)  

57.

stain for capsule

a)

Crystal Genshin

b)

Leifson, Gray

c)

Fisher and Conn

d)

HISS and India Ink

58.

stains for flagella

a)

Leifson

b)

Gray

c)

Hiss

d)

Fisher and Conn

59.

flagella of vibrio

(a)  

60.

tumbling motility

(a)  

61.

darting motility

(a)  

62.

gliding motility

(a)  

63.

commonly from gram negative bacteria

a)

endotoxin

b)

exotoxin

64.

do not require bacterial death for release

a)

endotoxin

b)

exotoxin

65.

Heat stable toxin

a)

endotoxin

b)

exotoxin

66.

causes DIC, fever, shock, hypotension

a)

endotoxin

b)

exotoxin

67.

Highly antigenic

a)

endotoxin

b)

exotoxin

68.

Fried rice poisoning

a)

bacillus cereus

b)

clostridium botulinum

69.

stains for spores

a)

schaeffer and fulton

b)

Grays and Leifsom

c)

Dorners and Wirtz and Conklin

d)

Hiss and India Ink

70.

select the organisms that have capsule

a)

h. parainfluenza

b)

N. gonorrhiae

c)

S. pneumoniae

d)

H. influenzae

e)

N. Mengitidis

71.

cell wall antigen

a)

O

b)

H

c)

K

72.

Flagell Antigen

a)

O

b)

H

c)

K

73.

Oxidase positive, Motile, Gram negative bacilli

(a)  

74.

all enterobacteriacae are motile except

a)

Salmonella and Shigella

b)

Shigella, Citrobacter

c)

Salmonella and Klebsiella

d)

Klebsiella and Shigella

75.

bacteria that have M protein

(a)  

76.

M protein

a)

S. pneumoniae

b)

S aureus

c)

S. pyogenes

d)

S. agalactiae

77.

common cause of food poisoning

a)

salmonella

b)

e coli

c)

s aureus

d)

shigella

78.

Select true

a)

V factor is produced by s. aureus thats why H. influenzae satellites around s. aureus

b)

H. influenzae and H. aegypticus require X and V factors

c)

H. ducreyi needs X only

79.

Select correct sequence in plated media

a)

Weigh - dissolve - dispense - sterilize

b)

sterilize - weigh - dissolve - dispense

c)

weigh - dissolve - sterilize - dispense

d)

dissolve - dispense - sterilize - weight

80.

Select correct sequence in tubed media

a)

Weigh - dissolve - dispense - sterilize

b)

sterilize - weigh - dissolve - dispense

c)

weigh - dissolve - sterilize - dispense

d)

dissolve - dispense - sterilize - weight

81.

Media that contains nutrient supplement for fastidious bacteria

a)

enrichment

b)

general isolation

c)

selective

d)

enriched

82.

Media that supports the growth of most nonfastidious bacteria

a)

enrichment

b)

general isolation

c)

selective

d)

enriched

83.

Media that contains inhibitors

a)

enrichment

b)

general isolation

c)

selective

d)

enriched

84.

media that enhances the growth of an organism

a)

enrichment

b)

general isolation

c)

selective

d)

enriched

85.

media that provide distinct colonial apperances of microorganisms

a)

enrichment

b)

differential

c)

selective

d)

enriched

86.

Select the odd one out

a)

Tryptic soy agar

b)

Lim Broth

c)

Nutrient Broth

d)

SDA

87.

Examples of Enriched media

a)

Lim, todd hewitt

b)

TSA, Nutrient Agar, SDA

c)

BAP, CAP

d)

MAC

88.

Examples of Enrichment

a)

Lim, todd hewitt, Thioglycollate

b)

TSA, Nutrient Agar, SDA, Thioglycollate

c)

BAP, CAP, LJ Medium

d)

MAC

89.

Organism that is enhanced by Alkaline peptone water

(a)  

90.

What is being enhanced by Tetrathionate broth

a)

Fungi

b)

Salmonella

c)

Mycobacterium, Nocardia

d)

Salmonella, shigella

91.

Enrichment broth for Salmonella and Shigella

a)

Alkaline Peptone Water

b)

NUtrient broth

c)

Selenite Broth

d)

Tetrathionate broth

92.

Inhibitors for gram positive bacteria

a)

potassium tellurite

b)

Crystal Violet

c)

sodium desoxycholate

d)

sodium azide

93.

Inhibitors for gram negative bacteria

a)

potassium tellurite

b)

Crystal Violet

c)

sodium desoxycholate

d)

sodium azide

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