WorksheetsMTB 2025
Total questions: 20
Worksheet time: 10mins
Question 1 Which characteristic is responsible for mycobacteria being strongly acid-fast when stained?
A. Being obligate aerobic bacilli
B. The presence of mycolic acid
C. Serpentine cord forms on media
D. Facultative intracellular growth
Question 2 What is the approximate slow generation time of mycobacteria, which helps prevent immune recognition?
A. 6 hours
B. 12 hours
C. 18 hours
D. 24 hours
Question 3 Which major virulence factor of M. tuberculosis is described as trehalose-6,6”-dimycolate?
A. Muramyl dipeptide
B. Wax D
C. Lipoarabinomannan (LAM)
D. Cord factor
Question 4 According to the pathogenesis of Tuberculosis, what event occurs in Stage V after caseous tubercles liquefy?
A. T lymphocytes begin to infiltrate
B. Spread of MTB into airways and other parts of the lung
C. Primary infection of non-immune alveolar macrophages
D. T-cell activation and liberation of gamma interferon
Question 5 Which of the following is a clinical feature associated with pulmonary tuberculosis infection?
A. Acute cough
B. Hemoptysis
C. Hypertension
D. Hyperthermia
Question 6 Which chemical agent is used in the laboratory to liquefy non-sterile specimens for decontamination prior to mycobacteria examination?
A. 2% NaOH
B. Malachite green
C. N-acetyl-L-cysteine (NALC)
D. Carbolfuchsin
Question 7 In the Ziehl-Neelsen staining technique, what color do non-acid-fast bacteria stain due to the counterstain?
A. Red
B. Green
C. Yellow-green
D. Blue
Question 8 Which stain utilizes Tergitol as a mordant (wetting agent/surfactant) and is performed without steaming?
A. Ziehl-Neelsen technique
B. Kinyoun stain
C. Fluoroescence stain
D. Gram stain
Question 9 According to the WHO reading interpretation for Ziehl-Neelsen stain, a result of 2+ indicates the presence of how many acid fast bacilli (AFB) per field?
A. 1–9 AFB/100 fields
B. 10–99 AFB/100 fields
C. 1–10 AFB per field in at least 50 fields
D. More than 10 AFB per field in at least 20 fields
Question 10 In the Runyon classification system based on pigment production, mycobacteria that produce pigment only when grown in the light are categorized as:
A. Scotochromogens
B. Rapid growers
C. Non-chromogens
D. Photochromogens
Question 11 What characteristic appearance do M. tuberculosis colonies typically exhibit when grown on Löwenstein-Jensen (LJ) slants?
A. Small, flat ‘dysgonic’ colonies
B. Off-white (buff) color, dry breadcrumb-like appearance
C. Coccoid or filamentous forms
D. Nonpigmented/light tan colonies
Question 12 The BACTEC 460 system detects mycobacterial growth rapidly by measuring which product released from the consumption of C14-labeled palmitic acid?
A. Radioactive CO2
B. Oleic acid
C. Increased fluorescence
D. Decreased pH
Question 13 Which diagnostic test differentiates members of the TB complex from Nontuberculous mycobacteria (NTM) based on the inhibition of TB complex growth in the presence of p-nitro-alpha-acetyl-amino-beta-hydroxypropriophenone?
A. Tuberculin skin test (TST)
B. Interferon-Gamma Release Assays (IGRA)
C. BACTEC NAP Test
D. Gene Xpert MTB-RIF
Question 14 Which result is conventionally positive for M. tuberculosis in biochemical testing?
A. Heat stable catalase
B. Reduction of nitrate to nitrite
C. Susceptibility to thiophene-2-carboxylic acid hydrazide (T2H)
D. Negative niacin accumulation
Question 15 Which drug used in the Directly Observed Treatment Short-course for Tuberculosis (DOTS-TB) primarily inhibits RNA synthesis?
A. Isoniazid (H)
B. Rifampicin (R)
C. Pyrazinamide (Z)
D. Ethambutol (E)
Question 16 The BCG vaccine used for tuberculosis prevention is derived from a live attenuated strain of which organism?
A. Mycobacterium leprae
B. Mycobacterium kansasii
C. Mycobacterium bovis
D. Mycobacterium avium
Question 17 Which Mycobacterium species is specifically listed as the causative agent for Swimming Pool Granuloma?
A. M. abscessus
B. M. ulcerans
C. M. marinum
D. M. xenopi
Question 18 Mycobacterium bovis is often acquired by humans through:
A. Direct close contact in crowded populations
B. Drinking unpasteurized milk from an infected cow
C. Aerosol transmission from highly symptomatic patients
D. Transmission via armadillos
Question 19 What is the principal target cell for Mycobacterium leprae in the human body, leading to nerve damage, anesthesia, and muscle paralysis?
A. Alveolar macrophage
B. T lymphocyte
C. Schwann cell
D. Endothelial cell
Question 20 In the clinical diagnosis of leprosy, patients with clinically active disease but who show no bacilli on slit-skin smear examination are categorized as having what type of disease?
Multibacillary disease
Lepromatous disease
Paucibacillary disease
Disseminated disease
