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WorksheetsGood pipetting practice
Total questions: 10
Worksheet time: 5mins
What is the advantage of using pipette?
Transport liquid with less precision
transport liquid with precision
The most common pipetting principle used in the lab is air displacement
True
False
Why do we prefer air displacement pipette?
tips used for air displacement are relatively inexpensive
Best used for handling aqueous solutions
Best used for handling viscuous solutions
tips are easier to change
Factors affecting air displacement pipette's accuracy are temperature, density, and altitude.
True
False
Select pipetting methods used for air displacement pipette.
Forward and reverse pipetting
Left and right pipetting
Vertical and horizontal pipetting
Forward and vertical pipetting
Which pipetting method does the picture refers to?
Vertical pipetting
Reverse pipetting
Inverse pipetting
Forward pipetting
Which liquids do we use reverse pipetting technique?
Buffer
Saline water
Glycerol
PCR products
Wrong tip selection will cause the following EXCEPT:
Accurate volume transferred
Malfunction due to accelerated wear of internal mechanical parts
Cross-contamination
Volume error due to loose-fit
For 1000uL pipette, staff should use 100uL pipette tip
True
False
Air displacement pipettes are exposed to cross-contamination due to these factors EXCEPT:
more liquid is aspirated than the tip volume allows
Aerosols from sample with virus or bacteria
Pipette turned upside down with a filled pipette tip
Using the right pipette tip
