WorksheetsPOST TEST – ELISA ASSAY
Total questions: 10
Worksheet time: 5mins
What is the main principle of the ELISA assay?
DNA hybridization
Enzyme-substrate reaction linked to antigen-antibody binding
Protein electrophoresis
Chemical precipitation
What does ELISA stand for?
Enzyme Linked Immunosorbent Assay
Enzyme Ligand Immuno-Solution Assay
Enzyme Labelled Interaction Study Assay
Enzyme Ligation and Immune Screening Assay
In an ELISA test, the color intensity produced is directly proportional to:
The temperature of incubation
The enzyme concentration only
The amount of target antigen or antibody in the sample
The amount of washing buffer used
Which component acts as the solid phase in ELISA?
A. Petri dish
B. Glass slide
C. 96-well microplate
D. Centrifuge tube
In the sandwich ELISA, what binds to the antigen first?
Secondary antibody
Capture antibody
Enzyme-substrate complex
What is the role of the blocking step in the ELISA workflow?
A. To increase enzyme activity
B. To prevent non-specific binding on the plate surface
C. To amplify color signal
D. To denature the antigen
Which enzyme is commonly used in ELISA assays?
Lipase
Amylase
Horseradish Peroxidase (HRP)
Polymerase
What is the purpose of constructing a standard curve in quantitative ELISA?
To calculate molecular weight of antigen
To determine the enzyme activity
To estimate antigen concentration in unknown samples
To check plate coating efficiency
Why is TGF-β measurement important in MSC analysis?
It indicates bacterial contamination
It reflects differentiation signaling and MSC potency
It measures the antibody concentration
It checks viability of cells after freezing
Choose the correct purpose of EGF testing using ELISA from the options below:
To evaluate EGF content and entrapment efficiency in transfersomes
To detect impurities in formulation
To measure microbial load
