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Christmas party

Total questions: 39

Worksheet time: 27mins

Name
Class
Date
1.

顏色如其名，細胞死活由它來宣判

a)

Trypan Blue

b)

Malachite Green

c)

Oil Red

d)

Crystal Violet

2.

看起來像火龍果汁，但碰到皮膚會灼傷，RNA extraction 就需要它

a)

Ethidium Bromide

b)

DMSO

c)

TRIzol

d)

Pitaya Juice

e)

SYBR Green

3.

Hint: It is commonly used in immunofluorescence and can make the cell nucleus light up.

(提示：四個英文字母、全大寫)

(a)  

4.

聖誕前夕，Samuel 與 Johnny 想放大出「Rudolph’s Red Gene（RRG）」的 DNA 片段，好讓馴鹿的鼻子在夜空中亮起紅光。他們完成 PCR 後，準備檢查反應是否成功，可利用哪些技術觀察結果？

a)

ELISA

b)

Real-time PCR

c)

Western blotting

d)

Agarose gel electrophoresis

5.

YCW Lab 中有做過 IL-33 相關的 project 有哪些人？

a)

侑恩

b)

薰

c)

品璇

d)

宏嘉

6.

Hint: TBS + ddH₂O + Tween 20

(提示：四個英文字母、全大寫)

(a)  

7.

In cell culture experiments, DMEM is one of the most commonly used media.

What does DMEM stand for?

a)

Distilled Molecular Enzyme Mixture

b)

Dulbecco’s Modified Eagle Medium

c)

Direct Mammalian Extraction Medium

d)

Double Membrane Enrichment Medium

8.

在YCW lab所拍攝過的合照當中，有一張合照的站位與此張照片相同，請問那張照片是在哪個地方所拍攝的？

a)

漢來海港餐廳

b)

實驗室

c)

台南大飯店

d)

醫學院大門口

9.

When subculturing adherent cells, adding too much (a)   can easily cause cell death.

(answer in all lowercase) (提示：七個英文字母、全小寫)

10.

做 Western blot 時，若照膠後看到整片都白的，沒有任何 band 出現，最可能的原因是？

a)

蛋白太多

b)

抗體太貴

c)

加錯二抗

d)

HRP 變成惡魔覺醒了

11.

Which of the following papers was not published by the YCW lab?

(以下哪一篇不是YCW lab所發表的paper?)

a)

Deubiquitinase USP24 activated by IL-6/STAT3 enhances PD-1 protein stability and suppresses T cell antitumor response

b)

Fully human chitinase-3 like-1 monoclonal antibody inhibits tumor growth, fibrosis, angiogenesis, and immune cell remodeling in lung, pancreatic, and colorectal cancers

c)

OPN promotes the aggressiveness of non-small-cell lung cancer cells through the activation of the RON tyrosine kinase

d)

IL-33/NF-κB/ST2L/Rab37 positive-feedback loop promotes M2 macrophage to limit chemotherapeutic efficacy in lung cancer

12.

Professor Yi-Ching Wang has received numerous awards —

which of the following awards has she received?

(請問王憶卿教授得過那些獎項？)

a)

2024 The National Science and Technological Council Designated Research Fellow Award

b)

66th Academic Award of Ministry of Education

c)

The 19th Tien Te Lee Biomedical Awards

d)

2018 K.T. Li Honorary Scholar Award, Taiwan

13.

依據目前的 YCW Lab 網站所示，珮嘉學姐的興趣是哪兩項？

a)

喝咖啡

b)

看動漫

c)

看韓劇

d)

喝酒

e)

看推理小說

14.

選出正確的 YCW Lab 直屬配對組合

a)

珮嘉-順宏

b)

侑恩-之浩

c)

倫翎/宏嘉-儷心

d)

薰-品璇

15.

Western blot 實驗中的 blocking step 常用？

a)

Milk

b)

Wine

c)

Distilled water

d)

PBS

16.

A: 我像夾娃娃機，專抓一個蛋白，抓到後再問它朋友是誰。

B: 我把細胞分成兩個王國，一個住有DNA的王冠，另一個住著大多數酵素。

請問 A & B 最有可能代表什麼實驗？

a)

A: IP-WB

B: Nuclear fractionation

b)

A: Nuclear fractionation

B: IP-WB

c)

A: IP-WB

B: qPCR

d)

A: IHC

B: Nuclear fractionation

17.

The 2025 Nobel Prize in Physiology or Medicine was awarded to?

a)

Susumu Kitagawa

b)

Mary E. Brunkow

c)

Shimon Sakaguchi

d)

Frederick J. Ramsdell

e)

Omar M. Yaghi

18.

老師的電子信箱為何？

a)

ycw5978@mail.ncku.edu.tw

b)

ycw5476@mail.ncku.edu.tw

c)

ycw5796@mail.ncku.edu.tw

d)

ycw5798@mail.ncku.edu.tw

19.

According to the current information on the YCW Lab website,

where did Dr. Yi-Ching Wang receive her PhD degree?

a)

University of Michigan

b)

Chung Shan Medical University

c)

Michigan State University

d)

National Taiwan University

20.

Samuel & Johnny 在實驗室加錯 buffer，結果產生綠色煙霧。請問最合理的下一步是？

a)

拍照打卡

b)

冷靜觀察

c)

呼喚PI

d)

召喚pipette力量

21.

Samuel 在測定 ELISA 時發現吸光值爆表，最合理的原因應該是？

a)

成功召喚了黃金惡魔

b)

substrate沒停反應

c)

machine特異性太高

22.

若 USP24 去除 ubiquitin chain，代表它具備何功能？

a)

acetyltransferase

b)

demethylase

c)

ligase

d)

deubiquitinase

23.

請輸入聖誕節祝福語

2 lines
24.

When PD-1 is overexpressed in T cells, they will:

(choose the best answer)

a)

Enter a transient rest phase for metabolic recovery

b)

Undergo hyperactivation and cause cytokine storm

c)

Become exhausted and lose cytotoxic function

d)

Differentiate into memory-like T cells with enhanced recall ability

25.

請解碼下列 RNA 序列。

UAUUGUUGG AUCUCU GCU UCUCAUCGUGAAUGGGAU GCUGAUGUUAUUUCUGAACGU

(全大寫回答、不需要標點符號；字母之間不需要空格，但單字之間需要空格)

(a)  

26.

Which of the following is NOT included in the main research areas of the YCW Lab?

a)

Anticancer drug/antibody development

b)

  • Transcription regulation and cell signaling

c)

Epigenetics and epigenomics

d)

Neuroendocrine tumor

e)

Vesicle trafficking and tumor microenvironment

27.

請將圖片中的剪影人物依照生日月份先後(1月→12月)排序

(先→後，以數字作答)

(a)  

28.

What is the main concept illustrated in the schematic diagram?

a)

Rab37 enhances cytotoxic T cell function through IL-6 inhibition and STAT3 inactivation.

b)

Rab37-mediated IL-6 secretion activates STAT3 to upregulate PD-1, forming an immunosuppressive TME.

c)

PD-1 signaling induces Rab37 expression to enhance autophagy and reduce tumor growth.

d)

IL-6 secretion by Rab37 directly suppresses CTLA-4 signaling to promote T cell activation.

29.

請將圖片中的剪影人物依照進入YCW Lab先後排序

(先→後，以數字作答)

(a)  

30.

According to the description in the passage, Johnny is most likely to use _____ as the experimental cell line.

a)

THP-1

b)

CE81T/VGH

c)

RAW 264.7

d)

MIA PaCa-2

e)

KYSE-510

31.

在這篇探討食道鱗狀上皮癌放化療抗性機制的研究中，研究團隊發現 SOX17 為 NRF2 的轉錄抑制因子，且 SOX17的啟動子甲基化導致其表現下調。為了逆轉此現象，他們開發出奈米製劑 ZVI@CMC。

你認為此藥物主要是透過哪一種機制發揮抗癌效果？ (提示：與表觀遺傳學相關)

a)

促進NRF2乙醯化以抑制氧化壓力反應

b)

抑制DNA甲基轉移酶，恢復SOX17表現

c)

阻斷SOX17與轉錄因子複合體的結合

d)

活化AKT/mTOR路徑以提升放療敏感性

32.

What is the main therapeutic strategy developed in this study?

a)

A small molecule inhibitor targeting CHI3L1

b)

A fully human monoclonal neutralizing antibody against CHI3L1

c)

An siRNA-based CHI3L1 silencing approach

d)

A CHI3L1-overexpressing vaccine

33.

According to the study, CHI3L1 nAbs exert anti-tumor effects mainly by?

a)

Activating fibroblasts and promoting angiogenesis

b)

Inducing macrophage-to-myofibroblast transformation

c)

Suppressing pancreatic stellate cell activation and reducing fibrosis

d)

Enhancing CHI3L1 secretion and immune suppression

34.

Researchers developed ​ _____-derived fully human monoclonal CHI3L1 antibodies.

a)

phage display

b)

yeast display

c)

ribosome display

35.

Which cancer types were primarily tested for CHI3L1 neutralizing antibody efficacy?

a)

Breast, prostate, and gastric cancers

b)

Lung, colorectal, and pancreatic cancers

c)

Liver, glioblastoma, and melanoma

d)

Ovarian, bladder, and renal cancers

36.

根據影片內容，老師說明求學問須包含哪些要點？

a)

Attitude

b)

Specific

c)

Knowledge

d)

Altitude

e)

Skill

37.

老師進行癌症研究最早使用的模式是：

a)

胰臟癌

b)

食道癌

c)

肺癌

d)

乳癌

38.

影片中有出現的醫師為？

a)

張維倫 醫師

b)

沈延盛 醫師

c)

沈孟儒 醫師

d)

蘇五洲 醫師

39.

影片中出現兩位憶卿老師的恩師，分別為？

a)

陳嘉芬 教授

b)

陳淑芬 教授

c)

Veronica Gaylie 教授

d)

Veronica Maher 教授

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