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WorksheetsPolymerase Chain Reaction Vocabulary
Total questions: 11
Worksheet time: 6mins
A laboratory technique used to make billions of copies of a specific segment of DNA
Polymerase Chain Reaction
Short DNA sequences that bind to specific parts of the target DNA sequence that tell the taq Polymerase where to start making a new strand of DNA
The specific DNA sequence of interest that is going to be amplified in PCR
Target DNA
Distinct sequence of DNA which codes for a specific protein
A DNA Polymerase found in bacteria that thrive around hot springs. It is used because it will not denature at high temperatures
The process of DNA denaturation, primer annealing and nucleotide extension. Each cycle copies one DNA sequence and creates two identical DNA sequences
At 75oC, taq Polymerase binds to the primer and adds DNA nucleotides to each complementary single stranded DNA
Annealing
At a temperature of around 55oC, DNA primers bind to the complementary sequences of the two single stranded DNA pieces. This is the second phase of the PCR Cycle.
A period of high temperature, 95°C, which unwinds the double-stranded DNA and creates two single-stranded DNA pieces. This is the first phase of the PCR Cycle
What happens during the anneal step of PCR?
Primers are created.
The primers attach to the target DNA region.
Primers copy the new DNA strand.
Primers sequence DNA
