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WorksheetsBacT review GPR and Neisseria
Total questions: 16
Worksheet time: 12mins
A wound culture grew dull grayish-white colonies with a "frosted glass" and "medusa head" appearance. The gram stain showed long, gram positive rods with endospores. This organism is expected to be:
Penicillin resistant
An anaerobe
Nonmotile
Beta hemolytic
Bacillus anthracis is mainly associated with all of the following EXCEPT:
Pulmonary anthrax
Gastrointestinal anthrax
Cutaneous anthrax
Urinary anthrax
Bacillus cereus is an aerobic, spore forming, GPR that is:
(Select all that apply)
Penicillin resistant
Motile
Catalase negative
Beta hemolytic
Lecithinase positive
True or False: All strains of Corynebacterium diphtheriae produce the diphtheria toxin.
True
False
Match the following:
Produces metachromatic granules, urease neg, brown halo and dark colonies on CTBA, causes white pseudomembrane in throat
Corynebacterium diphtheriae
Urease negative, nitrate reducing, PYR +, most common cause of prosthetic valve endocarditis
Corynebacterium jeikeium
Reverse CAMP+, produces brown halo on CTBA, usually veterinary pathogen but can cause granulomatous lymphadenitis
Corynebacterium pseudotuberculosis
Reverse CAMP+, Nitrate reduction (=), urease +, brown halo on CTBA, cause diphtheria-like illness in humans and mastitis in cattle
Corynebacterium ulcerans
Nitrate reduction (=), rapid urease +, most commonly associated with UTI's
Corynebacterium urealyticum
(a) is a short, non-branching GPR that causes meningitis in newborns, produces a narrow zone of beta hemolysis, is catalase +, CAMP + showing block type hemolysis, hippurate +, esculin hydrolyzer that is motile at 25C and shows an umbrella motility pattern with tumbling motility.
A 40-year-old veterinarian presents to the clinic with a 3-day history of mild swelling, itching, and a sharply demarcated patch on the right index finger. He reports getting scratched by a turkey recently. A wound culture was ordered, and it grew gray colonies with alpha hemolysis. A gram stain showed gram positive pleomorphic bacilli. The organism is most likely:
(select all that apply)
Catalase positive
H2S producer
Display test tube brush-like pattern on gelatin
(a) is a gram-positive bacillus that is beta hemolytic, non-motile, resistant to penicillin, reverse CAMP+, catalase =, is genetically similar to Trueperella spp., and can cause pharyngitis in patients 10-20 years of age.
Nocardia spp. are branching GPR that (select all that apply):
stain gram variable
are modified acid fast positive
prefer to grow on BCYE
are strict anaerobes
This organism is a gram-positive cocci/coccobacillus that can often be found in histiocytes, is modified acid fast positive, is a part of the actinomycetes, and produces salmon pink colonies:
Streptomyces spp.
Rhodococcus spp.
Actinomadura spp.
Gordonia spp.
Which of the following is a major virulence factor of Neisseria meningitidis?
Capsule
IgA protease
Pili
Major outer membrane porin proteins
All of the above
A 25-year-old female presents to the emergency room with an acute urethral discharge of two days duration. The image shows the Gram-stained smear that was obtained. Many polymorphonuclear (PMN) leukocytes and intracellular and extracellular gram-negative diplococci were observed. Based on the clinical history and the Gram stain observation, a diagnosis of gonorrhea can be made.
True
False
Which growth medium is most commonly used to culture Neisseria gonorrhoeae?
MacConkey agar
Blood agar
Chocolate agar
Thayer martin
_______, ______, and _______ are 3 biochemical tests we can perform to identify Neisseria gonorrhoeae.
(a)
A complication of disseminated Neisseria meningitidis infection is also known as: (a)
A 65-year-old patient with a history of chronic obstructive pulmonary disease presents with increased cough, purulent sputum, and shortness of breath. A sputum sample was sent to the lab for culture and grew round, yellow, "hockey puck" colonies. This organism is likely:
Catalase positive
Oxidase negative
A gram-negative rod
DNase negative
