WorksheetsAP Bio Enzymes Test
Total questions: 19
Worksheet time: 22mins
Which of the following statements best helps explain the reaction specificity of an enzyme?
the shape and charge of the substrates with the active site of the enzyme
the free energy of the reactants is greater than the free energy of the products
the equilibrium constant of the reaction is much greater than 1
the concentration of the enzyme inside living cells is greater than the concentration of substrate
which of the following is true about enzyme catalyzed reactions
all of these are true
the reaction rate is faster than the same reaction in the absence of the enzyme
they could occur without any activation energy needed
the products have more or less energy than the products of the same reaction in the absence of the enzyme
they use up the enzyme permanently changing it so that it is unusable
a series of enzymes catalyze the reaction X->Y->Z->A. product A bonds to the enzyme that converts X to Y at a position remote from its active site. this binding decreases the activity of the enzyme. the information above tells us that which of the following is true?
Y is both a substrate and a product
this is a metabolic pathway that functions as a negative feedback loop
A is a competitive inhibitor
X is a substrate
Alcohol dehydrogenase (ADH) is an enzyme that aids in the decomposition of ethyl alcohol into nontoxic substances. Methyl alcohol acts as a competitive inhibitor of ethyl alcohol by competing for the same active site on ADH. When attached to ADH, methyl alcohol, is converted to formaldehyde, which is toxic in the body. Which of the following statement statements, best predicts the effect of increasing the concentration of substrate (ethyl alcohol), while keeping the concentration of the inhibitor (methyl alcohol) constant?
there will be an increase in formaldehyde because ADH activity increase
competiitive inhibition will be terminated because ethyl alcohol will bind to methyl alcohol and decrease ADH activity
the peptide bonds in the active site of the enzyme will be denatured, inhibiting the enzyme
competitive inhibition will decrease because the proportion of the active sites occupied by the substrate will increase
Which of the following statements best justifies the inclusion of test tubes 3 and 7 in the experiment?
they will show the rate of product formation in the absence of bacterial factors
they will show the rate of product formation in the absence of the substrate
they will show whether environmental pH affects the function of the enzyme
they will show whether the isolated cellular contents have enzymatic activity
The structure above could be any of the following EXCEPT
molecule of adenosine triphosphate
monomer used as a component of a polypeptide
reactant used in the synthesis of a nucleic acid
molecule that is the energy source for most anabolic activity in cells
nucleotide
A researcher claims that only a portion of the light energy captured by green plants is available for growth and repair. Which of the following observations best helps justify the researchers claim?
light capturing pigment molecules in green plants, absorb red blue and violet light, but reflect green light
The energy of a photon of light is proportional to its frequency and inversely proportional to its wavelength
captured energy is stored in the molecular bonds of organic molecules, including simple sugars and starch
as light energy is converted to chemical energy by metabolic processes, some of the energy is lost as heat
A researcher claims that only a portion of the light energy captured by green plants is available for growth and repair.
Which of the following observations best helps justify the researcher's claim?
the three dimensional structure of the enzyme
the temperature of the aqueous buffer solution
the final concentration of the product
the initial concentration of hhe substrate
which of the following can be used to determine the rate of enzyme catalyzed reactions
rate of disappearance of the substrate
change in volume of the solution
increase of activation energy
rate of disappearance of the enzyme
rate of disappearance of the product
A group of AP Biology students is testing the effect of the amount of peroxidase enzyme on oxygen production. In cells, peroxidase breaks down hydrogen peroxide into water and oxygen gas. Identify an appropriate control group for this experiment.
the amount of peroxidase enzymes should be constant, making this the control group
The group in the highest temperature condition will serve as the control since it will slow the most extreme effects of heat
the amount of substrate is the control group since it will be kept the same to allow for a valid experiment
the group with the same name total volume, with water in place of catalyze enzyme
Which of the following best predicts how the structure and function of pepsin will change as it enters the small intestine?
pepsin will not change in shape but may not work due to the basic environment of the small intestine
Pepsin will not change in shape and will continue to breakdown proteins in the small intestine
Pepsin will change in shape because of the basic environment of the small intestine therefore, its enzymatic activity will decrease
pepsin will change in shape because of the presence of trypsin and chymotrypsin in the small intestine, both of which act as competitive inhibitors.
Graphs I-IV depict the effect of pH on the activity of four different hydronic enzymes. The most likely explanation for the results shown in graph I is that
the enzyme has disulfide bonds
pH affects the temperature of the reaction
pH affects the primary structure of the enzyme
pH affects the shape of the active site of the enzyme
the enzyme has a quaternary structure
The structure above could be any of the following EXCEPT
molecule of adenosine triphosphate
monomer used as a component of a polypeptide
reactant used in the synthesis of a nucleic acid
molecule that is the energy source for most anabolic activity in cells
nucleotide
increasing the substrate concentration in an enzymatic reaction could overcome which of the following?
allosteric inhibition
competitive inhibition
desaturation of the enzyme
saturation of the enzyme activity
A researcher claims that the synthesis of ATP from ADP and inorganic phosphate is essential to cellular function
Which of the following statements best helps justify the researcher's claim?
ADP is a small molecule that some cells release into their environment as a way of communication with other cells
ATP hydrolysis is an energy releasing reaction that is often coupled with reactions that require an input of energy
inorganic phosphate is a substance that cells typically acquire from their environment
ATP synthase is a mitochondrial enzyme that catalyzes the conversion of ADP and inorganic phosphate to ATP
Amylase is a protein that catalyzes the conversion of starch to simple sugars. Amylase activity in an aqueous solution can be measured by using iodine as a starch indicator. A solution containing iodine and starch will have a dark blue color, whereas a solution containing iodine, but no starch will have a light brown color. The color change of an iodine solution from dark blue to light brown can be used to measure the rate at which starch is converted to simple sugars. A student designs an experiment to investigate the effect of environmental pH on amylase function. The design of the experiment is presented in table 1. which of the following statements best justifies the inclusion of test tube V as a control in the experiment?
it will provide a measurement of amylase activity at a basic pH
it will show the color change that occurs in the absence of the amylase protein
it will show the color change that occurrs in the absence of enzyme activity
so called high energy bonds include the bonds at
D and E only
B and C only
C and D only
B, C, D, and E
A and B only
Graphs I-IV depict the effect of pH on the activity of four different hydrolic enzymes. Enzymes with their highest activity at an alkaline (basic) pH represented by which of th following graphs?
I only
II only
III only
I and III only
I and IV only
increasing the substrate concentration in an enzymatic reaction could overcome which of the following?
allosteric inhibition
competitive inhibition
desaturation of the enzyme
saturation of the enzyme activity
