WorksheetsQuiz: Case Studies about Protein Analysis and Immunoassay
Total questions: 12
Worksheet time: 16mins
Name
Class
Date
1.
Student ID (NIM)
4 lines
2.
NAME
4 lines
3.
The following interpretation of SPE results in multiple myeloma (MM) patients is:
a)
Increased albumin in MM
b)
Decreased albumin in MM
c)
Increased gamma protein in MM
d)
Decreased albumin and increased gamma protein in MM
e)
Increased albumin and gamma protein in MM
4.
The SPE method proposed in the journal ...
a)
Cellulose acetate with pH 8.6
b)
Agarose with pH 8.6
c)
Filter papers (Whatman) with pH 7.6
d)
Agarose with pH 7.6
e)
Cellulose acetate with pH 7.6
5.
The staining used in SPE used in the journal ...
a)
Comassie blue
b)
Amido black
c)
Ponceu S
d)
Nistatin
6.
The aim of the journal "Development of sandwich ELISA systems for the diagnosis of hepatitis B virus surface antigen and its antibodies in human sera" is
a)
Finding markers to support the diagnosis of hepatitis B
b)
Making an ELISA kit for the diagnosis of hepatitis B
c)
Testing for false results in hepatitis B diagnosis methods currently used commercially
d)
Produce monoclonal antibodies
7.
In the detection of anti-HBsAg, serum acts as...
a)
Antigen
b)
Antibody
8.
Principle of sandwich ELISA test for detecting HBsAg
a)
Anti-HBsAg antibody coated in microwell plate
b)
HBsAg from sample or control
c)
Anti-HBsAg antibody conjugated to HRP
d)
Enzyme activity is measured by adding TMB substrate buffer
e)
All true
9.
The immunoblot steps for Western Blot in Leishmaniasis identification is
a)
Antigen - Serum - Labeled antibody - Substrate
b)
Capture antibodies - Serum - Labeled antibodies - Substrate
c)
Antigen - Capture antibody - Serum - Labeled antibody - Substrate
d)
Capture antibody - Serum - Monoclonal antibody - Labeled antibody - Substrate
10.
The immunoblot type in Western Blot for Leishmaniasis is
a)
Direct Western Blot
b)
Indirect Western Blot
11.
The antibodies used in indirect ELISA for the diagnosis of tuberculosis are:
a)
monoclonal antibodies and labeled antibodies
b)
monoclonal antibodies
c)
labeled antibodies
d)
polyclonal antibodies and labeled antibodies
12.
The main method for detecting tuberculosis that is still used today is ...
a)
Acid-fast bacilli test from sputum specimen
b)
Bacterial culture with blood specimen
c)
Indirect ELISA
d)
Western blot
e)
Sandwich ELISA
100 %
