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FINAL: DNA Structure & Replication Class Worksheet #2—Test

Total questions: 43

Worksheet time: 22mins

Name
Class
Date
1.

Which three parts make up a nucleotide?

a)

Sugar, base, enzyme

b)

Phosphate, ribosome, base

c)

Phosphate, sugar, nitrogen base

d)

Sugar, lipid, base

2.

What is the basic unit (monomer) of DNA?

a)

Amino acid

b)

Nucleotide

c)

Protein

d)

Sugar

3.

What type of sugar is found in DNA?

a)

Ribose

b)

Glucose

c)

Deoxyribose

d)

Sucrose

4.

Which nitrogen base pairs with adenine in DNA?

a)

Cytosine

b)

Guanine

c)

Thymine

d)

Uracil

5.

Which base pair is held together by three hydrogen bonds?

a)

A–T

b)

T–A

c)

C–G

d)

A–C

6.

The sides (backbone) of the DNA ladder are made of:

a)

Nitrogen bases

b)

Hydrogen bonds

c)

Sugars and phosphates

d)

Proteins

7.

The rungs of the DNA ladder are made of:

a)

Sugar-phosphate bonds

b)

Complementary base pairs

c)

Phosphate groups

d)

Enzymes

8.

DNA strands run in opposite directions. This is described as:

a)

Parallel

b)

Identical

c)

Antiparallel

d)

Complementary

9.

What is the overall shape of DNA?

a)

Straight ladder

b)

Single helix

c)

Double helix

d)

Circular

10.

Which statement best describes complementary base pairing?

a)

Bases pair randomly

b)

A pairs with G and C pairs with T

c)

A pairs with T and C pairs with G

d)

All bases pair equally

11.

What is DNA replication?

a)

DNA being repaired

b)

DNA being translated

c)

DNA being copied

d)

DNA being condensed

12.

During which phase of the cell cycle does DNA replication occur?

a)

G1

b)

S

c)

G2

d)

M

13.

Which enzyme unwinds and “unzips” DNA during replication?

a)

DNA polymerase

b)

RNA polymerase

c)

DNA helicase

d)

Ligase

14.

What type of molecule is DNA helicase?

a)

Lipid

b)

Carbohydrate

c)

Enzyme

d)

Nucleotide

15.

What happens first during DNA replication?

a)

Free nucleotides attach

b)

Hydrogen bonds form

c)

Hydrogen bonds break

d)

New DNA strands join

16.

Free nucleotides attach to exposed DNA bases using:

a)

Random pairing

b)

Hydrogen bonding rules

c)

Complementary base pairing

d)

Sugar bonding

17.

Why is DNA replication called semi-conservative?

a)

DNA is only copied halfway

b)

One new strand is shorter

c)

Each new DNA has one original strand

d)

DNA is conserved in the nucleus

18.

Each new DNA molecule produced contains:

a)

Two original strands

b)

Two new strands

c)

One original and one new strand

d)

No original strands

19.

How does semi-conservative replication help prevent mutations?

a)

It shortens DNA

b)

It removes damaged DNA

c)

It uses original strands as templates

d)

It stops replication errors

20.

What holds newly formed base pairs together in DNA?

a)

Covalent bonds

b)

Ionic bonds

c)

Hydrogen bonds

d)

Peptide bonds

21.

What term describes loose DNA inside the nucleus?

a)

Chromosome

b)

Chromatin

c)

Sister chromatid

d)

Centromere

22.

When DNA is condensed and visible, it is called a:

a)

Chromatin fiber

b)

Chromosome

c)

Nucleotide

d)

Base pair

23.

The cell cycle is best described as:

a)

DNA replication only

b)

Cell division only

c)

A repeating process of growth and division

d)

Protein synthesis

24.

Which stages together make up interphase?

a)

G1, S, G2

b)

S and M

c)

G2 and M

d)

Prophase and metaphase

25.

During which phase does the cell grow and carry out normal functions?

a)

S

b)

G1

c)

G2

d)

M

26.

What happens during G2 phase?

a)

DNA replicates

b)

Cell divides

c)

Cell prepares for division

d)

Chromosomes separate

27.

Sister chromatids are:

a)

Two different chromosomes

b)

Identical copies of a chromosome

c)

Unreplicated DNA

d)

RNA molecules

28.

What structure holds sister chromatids together?

a)

Spindle fiber

b)

Hydrogen bonds

c)

Centromere

d)

Ribosome

29.

Replicated chromosomes are often drawn as an X shape because:

a)

They are twisted

b)

They are broken

c)

They consist of two sister chromatids

d)

They are circular

30.

During which phase do chromosomes first become visible?

a)

S phase

b)

G1 phase

c)

Prophase

d)

G2 phase

31.

According to Chargaff's Rule, if a DNA sample is 30% adenine, thymine will be:

a)

20%

b)

30%

c)

40%

d)

60%

32.

If cytosine makes up 22% of a DNA sample, guanine will be:

a)

11%

b)

22%

c)

44%

d)

78%

33.

Why do base percentages vary between species?

a)

DNA shape changes

b)

Number of chromosomes differs

c)

Genetic sequences are different

d)

Base-pair rules change

34.

Which relationship is always true in DNA?

a)

A = C

b)

T = G

c)

A = T

d)

A = G

35.

Base-pair rules help ensure:

a)

DNA stays short

b)

DNA mutates frequently

c)

Accurate DNA replication

d)

DNA forms RNA

36.

During DNA replication, what is the role of the template strand?


a)

It joins Okazaki fragments

b)

It provides the pattern for building a new strand

c)

It adds RNA primers

d)

It seals DNA fragments

37.

Semi-conservative replication means that:


a)

DNA is copied only once

b)

Each new DNA molecule contains one original strand and one new strand

c)

Only half the DNA is copied

d)

DNA is conserved in the nucleus

38.

Why does complementary base pairing help ensure accurate DNA replication?


a)

It speeds up protein synthesis

b)

It prevents helicase from working

c)

It allows correct nucleotides to match the template strand

d)

It forms RNA primers

39.

Which feature is shared by BOTH DNA and RNA?


a)

Both contain thymine

b)

Both are double-stranded

c)

Both are made of nucleotides

d)

D. nucleus

40.

Which statement best describes how DNA, RNA, and ribosomes work together to make proteins?


a)

DNA leaves the nucleus and builds proteins on ribosomes

b)

RNA stores genetic information permanently

c)

RNA carries instructions from DNA to ribosomes, where proteins are made

d)

Ribosomes copy DNA into RNA

41.

Why is the lagging strand synthesized in fragments?


a)

DNA polymerase moves randomly

b)

DNA polymerase only works in the 5′ → 3′ direction

c)

Helicase skips sections

d)

Primase stops early

42.

 Okazaki fragments are:


a)

RNA primers

b)

Short DNA segments on the lagging strand

c)

Enzymes

d)

Hydrogen bonds

43.

Which sequence correctly shows the order of events during DNA replication?


a)

DNA polymerase → primase → helicase

b)

Primase → helicase → DNA polymerase

c)

Helicase → primase → DNA polymerase

d)

DNA ligase → helicase → primase