Understanding CRISPR for Students

Front

CRISPR full form

Back

Clustered Regularly Interspaced Short Palindromic Repeats

Front

CRISPR-Cas9

Back

A genome editing tool

Front

Cas9

Back

An enzyme that cuts DNA

Front

Guide RNA (gRNA)

Back

Directs Cas9 to specific DNA sequence

Front

PAM sequence

Back

Protospacer Adjacent Motif, necessary for Cas9 binding

Front

Double-strand break

Back

DNA cut by Cas9

Front

Limitations of CRISPR

Back

difficult to deliver; uses viral vectors; may not be efficient; may not be accurate

Front

How do bacteria use CRISPR?

Back

defense mechanism; destroy the invader

Front

CRISPR origin

Back

Bacterial immune system

Front

CRISPR applications

Back

Gene editing, disease research, agriculture