PCR Review Flashcard
Flashcard
•
Science
•
12th Grade
•
Practice Problem
•
Hard
Noha Salah
FREE Resource
Student preview

30 questions
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1.
FLASHCARD QUESTION
Front
Describe the process of PCR amplification.
Back
PCR amplification is a technique used to make multiple copies of a specific DNA segment.
2.
FLASHCARD QUESTION
Front
What is the purpose of PCR?
Back
To produce millions of copies of a specific region of DNA
3.
FLASHCARD QUESTION
Front
Steps involved in the Polymerase Chain Reaction process.
Back
Denaturation, Annealing, and Extension
4.
FLASHCARD QUESTION
Front
What is the significance of the annealing step in the PCR process?
Back
It allows the primers to bind to the DNA template.
5.
FLASHCARD QUESTION
Front
What is the denaturation step?
Back
separating DNA helix with using heat
6.
FLASHCARD QUESTION
Front
At what temperature does the Denature step of PCR occur?
Back
94-96° C
7.
FLASHCARD QUESTION
Front
Factors to consider when designing PCR primers.
Back
Primer length, GC content, melting temperature, specificity, and avoiding self-complementarity
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