MBT FL24 Transformation

Front

What is transformation in the context of bacterial cells?

Back

Transformation is the insertion of a foreign plasmid into a bacterial cell, resulting in new acquired traits.

Front

What is the correct sequence of steps in the genetic transformation process?

Back

1. Bacterial cells are transferred to cold CaCl2. 2. Plasmid with foreign DNA is added to the bacteria. 3. Cells are heat shocked at 42°C. 4. Cells are spread on an agar petri dish. 5. Bacteria are given time to grow on the petri dish.

Front

Why is ampicillin used in transformation plates?

Back

Ampicillin is used to isolate and select for only the bacteria that have been successfully transformed.

Front

Which agar plate would show no bacterial growth?

Back

The pGLO LB/amp plate would show no bacterial growth.

Front

What process is used to increase the competency of bacterial cells?

Back

The competency of cells is increased through the use of calcium chloride and heat shock.

Front

What is a plasmid?

Back

A plasmid is a small, circular piece of DNA that can replicate independently of chromosomal DNA in bacteria.

Front

What role does calcium chloride play in transformation?

Back

Calcium chloride helps to make bacterial cells more permeable to DNA, facilitating the uptake of plasmids.

Front

What is heat shock in the context of transformation?

Back

Heat shock is a brief exposure of bacterial cells to elevated temperatures, which helps to increase the uptake of plasmid DNA.

Front

What is the purpose of spreading transformed bacteria on an agar plate?

Back

Spreading transformed bacteria on an agar plate allows for the growth of individual colonies, which can be selected based on antibiotic resistance.

Front

What is the significance of antibiotic resistance in transformed bacteria?

Back

Antibiotic resistance indicates that the bacteria have successfully taken up the plasmid containing the resistance gene, allowing them to survive in the presence of the antibiotic.