Biotechnology HW 8.6

Front

What is the final step in the miniprep procedure before storing the plasmids?

Back

Resuspending the plasmid pellet in TE buffer.

Front

What is the purpose of using restriction enzymes in the extracted plasmid sample?

Back

To confirm plasmid size and sequence.

Front

What is the purpose of adding isopropanol during the miniprep procedure?

Back

To precipitate nucleic acids.

Front

What is one advantage of using commercial kits for plasmid preparation?

Back

They provide pre-made buffers and solutions.

Front

What is the name of the procedure in which plasmids are extracted from cells?

Back

Plasmid prep.

Front

What is a plasmid?

Back

A small, circular piece of DNA that is separate from chromosomal DNA and can replicate independently.

Front

What is TE buffer and its role in plasmid storage?

Back

TE buffer is a solution of Tris-EDTA that helps to stabilize DNA during storage.

Front

What are restriction enzymes and how do they function?

Back

Restriction enzymes are proteins that cut DNA at specific sequences, allowing for analysis and manipulation of DNA.

Front

What is the significance of plasmid size in molecular biology?

Back

Plasmid size can affect the efficiency of transformation and the expression of inserted genes.

Front

What is the role of isopropanol in nucleic acid purification?

Back

Isopropanol is used to precipitate nucleic acids from solution, allowing for their collection.