Restriction Enzymes

Front

What is a restriction enzyme?

Back

An enzyme that cuts DNA molecules at specific recognition sites.

Front

Where are restriction enzymes isolated from?

Back

Bacteria.

Front

What is the complementary sequence of GCTTCG?

Back

CGAAGC.

Front

What are the unpaired nucleotides produced by restriction enzymes called?

Back

Sticky ends.

Front

How many fragments does a restriction enzyme cut DNA into if it recognizes a sequence and cuts it?

Back

It depends on the number of recognition sites; for one cut, it produces 2 fragments.

Front

What is the function of restriction enzymes in molecular biology?

Back

To cut DNA at specific sequences for cloning, analysis, or modification.

Front

What are sticky ends?

Back

Single-stranded ends of DNA that are generated by the action of restriction enzymes.

Front

What is the significance of recognition sites for restriction enzymes?

Back

They are specific sequences where restriction enzymes bind and cut DNA.

Front

Can restriction enzymes be used in genetic engineering?

Back

Yes, they are essential tools for cutting and pasting DNA in genetic engineering.

Front

What is the difference between sticky ends and blunt ends?

Back

Sticky ends have overhanging single-stranded DNA, while blunt ends are straight cuts with no overhang.