PCR Genetic testing

Front

What does PCR stand for?

Back

Polymerase Chain Reaction.

Front

What is the primary purpose of PCR?

Back

To produce millions of copies of a specific region of DNA.

Front

True or False: PCR copies all the DNA in an organism.

Back

False.

Front

What is the first step in a PCR cycle?

Back

Heating the DNA to 95 degrees C to separate the two complementary strands.

Front

What happens during the annealing phase of PCR?

Back

Primers attach to the target DNA region.

Front

What is the role of primers in PCR?

Back

To identify the particular region of DNA to be copied by PCR.

Front

What temperature is typically used to denature DNA in PCR?

Back

95 degrees C.

Front

What is the function of DNA polymerase in PCR?

Back

To synthesize new DNA strands by adding nucleotides.

Front

During which phase of PCR does DNA synthesis occur?

Back

The extension phase.

Front

What is the typical temperature for the extension phase in PCR?

Back

Around 72 degrees C.