Restriction Enzymes

Front

What is the complementary sequence of GCTTCG?

Back

CGAAGC

Front

What do restriction enzymes do to DNA?

Back

Cut

Front

How many fragments are produced when a restriction enzyme cuts DNA at one recognition site?

Back

2

Front

What are the unpaired nucleotides produced by restriction enzymes called?

Back

Sticky ends

Front

What is an enzyme that cuts DNA at a specific recognition site called?

Back

Restriction enzyme

Front

What is the role of restriction enzymes in genetic engineering?

Back

They cut DNA at specific sequences, allowing for the manipulation of genes.

Front

What are restriction fragments?

Back

Pieces of DNA that result from the cutting action of restriction enzymes.

Front

What is a recognition site in the context of restriction enzymes?

Back

A specific sequence of nucleotides where a restriction enzyme cuts the DNA.

Front

Can restriction enzymes be used to join DNA fragments?

Back

No, they are used to cut DNA, but other enzymes like ligases are used to join them.

Front

What is the significance of sticky ends in DNA manipulation?

Back

They allow for the easy joining of DNA fragments from different sources.