RECOMBINANT DNA TECHNOLOGY

Front

What is recombinant DNA technology?

Back

A method used to combine DNA from different sources to create new genetic combinations.

Front

What is a restriction endonuclease?

Back

An enzyme that cuts DNA at specific sequences, allowing for the manipulation of genetic material.

Front

What is the purpose of PCR (Polymerase Chain Reaction)?

Back

To amplify a specific segment of DNA, making millions of copies for analysis.

Front

What happens during the Denature step of PCR?

Back

The double-stranded DNA is separated into two single strands of DNA.

Front

What is the Anneal step in PCR?

Back

Primers attach to the target DNA region to initiate replication.

Front

What is a plasmid?

Back

A small, circular piece of DNA that can replicate independently of chromosomal DNA in bacteria.

Front

What are restriction sites?

Back

Specific sequences in DNA where restriction enzymes cut.

Front

What is a sticky end?

Back

A type of DNA end that has overhanging nucleotides, allowing for easier ligation with other DNA fragments.

Front

What is a blunt end?

Back

A type of DNA end that is straight-cut with no overhanging nucleotides.

Front

What is the role of DNA ligase in recombinant DNA technology?

Back

An enzyme that joins DNA fragments together by forming phosphodiester bonds.