MI 1.4 Practice Flashcard

Front

What is the significance of the recognition sequence for a restriction enzyme?

Back

It determines the enzyme's ability to cut DNA.

Front

Which type of vaccine uses a weakened version of the pathogen?

Back

Live attenuated.

Front

How many cuts are necessary when using restriction enzymes to cut viral/donor DNA and bacterial plasmid?

Back

One cut in the plasmid, two cuts in the viral DNA.

Front

What is a conjugate vaccine?

Back

A conjugate vaccine is a vaccine created by attaching pieces from the coats of bacteria to a carrier protein.

Front

What is a pathogen?

Back

A pathogen is a microorganism that causes disease.

Front

What are restriction enzymes?

Back

Restriction enzymes are proteins that cut DNA at specific sequences.

Front

What is the role of a plasmid in genetic engineering?

Back

A plasmid is a small circular DNA molecule used as a vector to transfer genetic material.

Front

What is the difference between live attenuated and inactive vaccines?

Back

Live attenuated vaccines use a weakened form of the pathogen, while inactive vaccines use killed pathogens.

Front

What is the function of a carrier protein in a conjugate vaccine?

Back

The carrier protein enhances the immune response to the attached bacterial pieces.

Front

What is the purpose of using restriction enzymes in genetic engineering?

Back

To cut DNA at specific sites for cloning or modifying genes.