Gel Electrophoresis and Cloning Review

Front

What is gel electrophoresis?

Back

A technique used to separate DNA fragments based on size.

Front

Why do DNA fragments move in gel electrophoresis?

Back

DNA is negatively charged, so it is attracted to the positive end of the gel.

Front

What determines the distance DNA fragments travel in gel electrophoresis?

Back

The size of the DNA fragments; smaller fragments travel farther than larger ones.

Front

What is the role of agarose gel in gel electrophoresis?

Back

Agarose gel acts as a medium through which DNA fragments can move, separating them by size.

Front

What is the purpose of using a DNA ladder in gel electrophoresis?

Back

A DNA ladder serves as a size reference to determine the size of unknown DNA fragments.

Front

What is cloning in the context of genetics?

Back

Cloning is the process of creating a genetically identical copy of an organism or DNA.

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What is a vector in genetic engineering?

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A vector is a DNA molecule used to deliver genetic material into a host cell.

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What is the significance of restriction enzymes in cloning?

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Restriction enzymes cut DNA at specific sequences, allowing for the insertion of DNA fragments into vectors.

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What is the outcome of successful gel electrophoresis?

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The outcome is a visual representation of DNA fragments, allowing for analysis and comparison.

Front

How can gel electrophoresis be used in forensic science?

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It can be used to compare DNA samples from crime scenes to suspects.