DNA Fingerprinting Flashcard

Front

What is a DNA fingerprint?

Back

A unique pattern made from fragments of a person’s DNA for comparison.

Front

Why is it necessary to perform PCR before gel electrophoresis?

Back

To make many copies of the DNA so that it is able to be seen.

Front

Whose blood was found in the blood stain at the crime scene?

Back

John.

Front

If opposite charges attract, what charge must the DNA have in order to move away from the wells?

Back

Negative.

Front

What is a restriction enzyme?

Back

A protein that cuts DNA at a specific place.

Front

What is the purpose of gel electrophoresis in DNA analysis?

Back

To separate DNA fragments based on their size.

Front

What role does DNA polymerase play in PCR?

Back

It synthesizes new DNA strands by adding nucleotides.

Front

What is the significance of using primers in PCR?

Back

Primers are short sequences that initiate DNA synthesis.

Front

How does DNA profiling help in forensic science?

Back

It allows for the identification of individuals based on their unique DNA patterns.

Front

What is the function of a DNA ladder in gel electrophoresis?

Back

It serves as a reference to determine the size of DNA fragments.