Protein Analysis and Chromatography Concepts

Front

What is a phosphate buffer?

Back

A phosphate buffer is a solution that resists changes in pH when small amounts of acid or base are added, commonly used in biological assays.

Front

What pH values are effective for phosphate buffers?

Back

Phosphate buffers are effective at pH values around 2.2, 7.2, and 12.4.

Front

What is the significance of ions in biological processes?

Back

Ions are crucial for various biological processes, including enzyme activity, nerve impulse transmission, and maintaining cell structure.

Front

What happens to electrostatic interactions in water?

Back

In water, the attraction of positive and negative charges is reduced, making electrostatic interactions significantly weaker.

Front

What is the charge of the phosphodiester backbone of DNA?

Back

The phosphodiester backbone of DNA is negatively charged.

Front

What is the role of a primary antibody in ELISA and Western blot?

Back

A primary antibody specifically interacts with a particular target molecule.

Front

What is the function of a secondary antibody in these assays?

Back

A secondary antibody binds to the primary antibody and is often conjugated to a detection enzyme or label.

Front

What is gel electrophoresis used for?

Back

Gel electrophoresis is used to separate proteins or nucleic acids based on their size and charge.

Front

How can protein contamination with nucleic acid be detected?

Back

By determining the absorbance at 260 nm (nucleic acids) vs 280 nm (proteins).

Front

What type of amino acid is rarely found at the N-terminal end of an alpha helix?

Back

Lysine is rarely found at the N-terminal end of an alpha helix.