MI 2.1.3 Review

Front

What is the optimal temperature for incubating a restriction digest?

Back

37°C

Front

What is the purpose of the annealing step in PCR?

Back

To allow primers to bind to their target complements.

Front

What correlation is investigated in the final module of the experiment?

Back

The correlation between genotype and the ability to taste PTC.

Front

What is the role of centrifuging in DNA isolation from cheek cells?

Back

To separate the cell debris from the DNA.

Front

What is the temperature range used during the denaturation step of PCR?

Back

94°C.

Front

Define restriction digest.

Back

A process that cuts DNA into smaller fragments using restriction enzymes.

Front

What is PCR and its primary purpose?

Back

Polymerase Chain Reaction (PCR) is a technique used to amplify DNA.

Front

Explain the role of primers in PCR.

Back

Primers are short sequences of nucleotides that provide a starting point for DNA synthesis.

Front

What is gel electrophoresis used for?

Back

To separate DNA fragments based on their size.

Front

Define DNA concentration.

Back

The amount of DNA present in a given volume of solution.