Biotechnology Semester 1 Final Practice

Front

What volume does the p200 dial pick up when set to 76?

Back

76 \mu L

Front

Order the following units from smallest to biggest: micro, milli, kilo.

Back

micro --> milli --> kilo

Front

What temperature is used in the extending step of a PCR cycle?

Back

70 C

Front

What is the purpose of DNA polymerase in a PCR cycle?

Back

To build out the new sequences, starting from the primers.

Front

What indicates a known mutation in ARMS-PCR?

Back

The mutant primers bind to the DNA while the wild-type primers do not.

Front

What is the main difference between STR and VNTR?

Back

STRs are 2-9bp long per repeat while VNTRs are 10-100bp long per repeat.

Front

What is the function of primers in PCR?

Back

Primers attach to the template DNA strands to initiate replication.

Front

Define STR in the context of genetics.

Back

Short Tandem Repeats, which are 2-9 base pairs long.

Front

Define VNTR in the context of genetics.

Back

Variable Number Tandem Repeats, which are 10-100 base pairs long.

Front

What is the role of gel electrophoresis in DNA analysis?

Back

To separate DNA fragments based on size.