MI U4 GFP Lab Mod 4

Front

What is a plastic cassette in the context of gel electrophoresis?

Back

A plastic cassette is a device that holds the gels used in gel electrophoresis, structured like a sandwich of plastic-gel-plastic.

Front

Does denatured GFP glow under UV light?

Back

No, denatured GFP does not glow under UV light.

Front

Why must gels be handled with care?

Back

Gels are very delicate and can be easily damaged, requiring extreme care during and after removal from the cassette.

Front

What is one method to denature proteins?

Back

Boiling them is a common method to denature proteins.

Front

Is staining the gels a one-step process?

Back

No, staining the gels typically involves multiple steps, not just covering with stain and draining excess.

Front

What is gel electrophoresis used for?

Back

Gel electrophoresis is used to separate and analyze macromolecules like DNA, RNA, and proteins based on their size and charge.

Front

What happens to proteins when they are denatured?

Back

Denatured proteins lose their natural structure and function, which can affect their behavior in experiments.

Front

What is the role of lysis buffer in protein denaturation?

Back

Lysis buffer helps to break down cell membranes and denature proteins, facilitating their extraction for analysis.

Front

What is the purpose of using UV light in GFP experiments?

Back

UV light is used to visualize GFP, as it fluoresces under UV illumination.

Front

What precautions should be taken when handling gels?

Back

Gels should be handled gently to avoid breaking or tearing, and should be kept on a stable surface.