Recombinant DNA

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What is recombinant DNA?

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Recombinant DNA is a form of DNA that is created by combining DNA sequences from different sources, often using biotechnology techniques.

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What is the first step in the process of recombining DNA?

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The first step is isolating the DNA from the organism of interest.

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What is the role of restriction enzymes in recombinant DNA technology?

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Restriction enzymes cut DNA at specific sequences, allowing scientists to isolate and manipulate DNA fragments.

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What is a cloning vector?

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A cloning vector is an agent, such as a plasmid, used to transfer foreign DNA into a host cell.

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What is the second step in the process of recombining DNA?

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The second step is cutting the DNA with a restriction enzyme.

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What is the third step in the process of recombining DNA?

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The third step is mixing the DNAs together and joining them using DNA ligase.

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What is the final step in the process of recombining DNA?

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The final step is allowing the bacterium to reproduce, which creates copies of the recombinant DNA.

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What is DNA ligase?

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DNA ligase is an enzyme that joins together DNA fragments by forming phosphodiester bonds.

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What is a plasmid?

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A plasmid is a small, circular piece of DNA that can replicate independently of chromosomal DNA in bacteria.

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What is the purpose of inserting recombinant plasmids into host cells?

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The purpose is to allow the host cells to express the foreign gene and produce the desired protein.