Biotechnology 4.4 HW

Front

What is Ethidium bromide (EtBr)?

Back

A DNA stain that is most commonly used and considered the most sensitive to low concentrations of DNA.

Front

What does a 1% agarose gel refer to?

Back

The concentration of agarose in the gel.

Front

What is the charge of RNA molecules during gel electrophoresis?

Back

Negative.

Front

What is agarose?

Back

A carbohydrate derived from seaweed used as a medium for horizontal gel electrophoresis.

Front

What is the primary use of gel electrophoresis?

Back

To separate charged molecules such as DNA, RNA, and proteins.

Front

What is the role of DNA stains in gel electrophoresis?

Back

To visualize DNA bands after electrophoresis.

Front

What is the purpose of using agarose in gel electrophoresis?

Back

To create a gel matrix that allows for the separation of nucleic acids based on size.

Front

How does the size of DNA fragments affect their movement in gel electrophoresis?

Back

Smaller DNA fragments move faster through the gel than larger ones.

Front

What is the significance of the voltage applied during gel electrophoresis?

Back

It affects the speed of migration of the charged molecules through the gel.

Front

What safety precautions should be taken when using Ethidium bromide?

Back

Wear gloves and goggles, and handle it in a fume hood due to its mutagenic properties.