Flashcards- ABE Lab 5a-6
Flashcard
•
Science
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9th Grade
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Practice Problem
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Easy
Kelly Jones
Used 2+ times
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27 questions
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1.
FLASHCARD QUESTION
Front
LAB 5a: Transforming Bacteria with the pARA-R Plasmid
Back
Introduce the genetically modified plasmid into E. coli bacteria to produce the rfp protein.
2.
FLASHCARD QUESTION
Front
Contents of the P+ tube
Back
The P+ tube contains the plasmid (and E. coli).
3.
FLASHCARD QUESTION
Front
Contents of the P- tube
Back
The P- tube does not contain the plasmid (but it did contain E. coli).
4.
FLASHCARD QUESTION
Front
Step causing plasmid entry into bacteria (transformation step)
Back
Heat shock causes the plasmid to enter the bacteria.
5.
FLASHCARD QUESTION
Front
Identifying transformed bacteria on petri plates
Back
Transformed bacteria can be identified by their growth on a plate that contains antibiotics. (untransformed cells would die)
6.
FLASHCARD QUESTION
Front
Environmental needs for transformed bacteria to make rfp protein
Back
Transformed bacteria need Arabinose (an inducer) in their environment to make rfp protein.
7.
FLASHCARD QUESTION
Front
Description of Recombinant plasmid of interest: pARA-R
Back
The pARA-R construct is a recombinant plasmid with a size of 5,302 base pairs. It includes sections such as araC, AmpR, PBAD, and rfp, with restriction sites BamHI and HindIII.
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