Flashcards- ABE Lab 5a-6

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LAB 5a: Transforming Bacteria with the pARA-R Plasmid

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Contents of the P+ tube

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The P+ tube contains the plasmid (and E. coli).

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Contents of the P- tube

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The P- tube does not contain the plasmid (but it did contain E. coli).

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Step causing plasmid entry into bacteria (transformation step)

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Heat shock causes the plasmid to enter the bacteria.

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Identifying transformed bacteria on petri plates

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Environmental needs for transformed bacteria to make rfp protein

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Transformed bacteria need Arabinose (an inducer) in their environment to make rfp protein.

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Description of Recombinant plasmid of interest: pARA-R

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What makes our E. coli competent?

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The have been coated in Calcium ions (which leads to adhesion zones allowing for the plasmids to potentially enter)

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Expected Growth of bacteria on plate containing only Luria Broth (LB plate)

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Expected Growth of bacteria on plate containing Luria Broth & Ampicillin (LB/amp plate)

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