An ELISA designed to detect the presence of antigens in a solution (usually a sample from a patient)

To remove a substance from a chromatography column by rinsing the column with a solvent

The process of separating one molecule from another by rinsing it with a solvent

enzyme linked immunosorbent assay (ELISA)

A technique that utilizes chemical specificity to detect and quantify a specific protein in a solution (usually a sample from a patient)

The removal of a specific molecule from a solution or preparation

A sample collected during chromatography elution

The state of having a high affinity (attraction) for water molecules

The state of being repelled by water molecules

hydrophobic interaction chromatography (HIC)

Separation of molecules based on their hydrophobicity

An ELISA designed to detect the presence of antibodies in a solution (usually a sample from a patient)

ion exchange chromatography

Separation of ions and polar molecules based on their affinity to an ion exchanger

A protein identification technique that utilizes a protein-copper chelation which results in reduced copper producing a color change

PAGE (polyacrylamide gel electrophoresis)

A technique that utilizes a vertical electrophoresis chamber to separate proteins by charge and size while the protein is in the native state

A stain a gel is soaked in after electrophoresis to allow visualization of bands (ex. Fast blast, coomassie blue, ethidium bromide)

Adding a stain to a sample or gel prior to electrophoresis (ex. syber green, ethidium bromide, EZ Vision) to allow visualization of samples

protein assay (general)

A laboratory technique to identify or quantify proteins in a sample

A method of isolating DNA or plasmids from bacteria by way of a column

Free floating nucleotides used in DNA Replication and PCR

The expected number of hits to see by chance when searching a sequence database; decreases exponentially as the Score (S) of the match increases

Using an electrical current in a buffer to split a molecule

The use of electric current to separate molecules based on size and charge through a solid media, such as agarose or polyacrylamide

Electrophoresis chamber

A container that transmits a controlled electric current through a solution to separate molecules located inside a gel

A segment of a gene that is transcribed and translated or fully expressed as a protein

Extension/elongation stage (PCR)

The third stage of PCR when mid-range temperature allows Taq polymerase to bond the complementary bases

A database for drosophila genetics and molecular biology

The primer that binds to the start codon on the template strand during PCR

The plastic container where a gel is molded

The use of proteins and nucleic acid to turn a gene on or off

The genetic material in an organism

A peer-reviewed genome sciences journal

The chromosomal DNA that makes up an organism's genome, does not include plasmids

The enzyme in DNA Replication that breaks the hydrogen bonds between base pairs

A gene sequence that has the same origin or function as another

A segment of a gene that is transcribed but not translated

A segment of DNA that is reversed end to end

Ladder/standard/marker

A sample of DNA or protein with known sizes, used to create a standard curve to determine the unknown size of DNA or protein samples

The enzyme that repairs nicks on the lagging strand during DNA Replication

A dye that increases the density of a DNA or protein sample to help it fall into the well, also provides a visual (dye front) of a known fragment size to monitor DNA migration

Ma'am-Gilbert sequencing

Using chemical modification of DNA followed by cleavage at bases complementary to modified nucleotides

A solution of Taq polymerase, dNTPs, and all the reagents necessary for PCR

MEGA (molecular evolutionary genetics analysis)

A tool for conducting automatic and manual sequence alignment, inferring phylogenetic trees, mining web-based databases, estimating rates of molecular evolution, and testing

The National Center for Biotechnology Information advances science and health by providing access to biomedical and genomic information

Next generation sequencing

Any of a variety of high throughput sequencing methods; Sanger and Maxam Gilbert considered the First Generation

Standards 9, 11, 12 Flashcards

Student preview

50 questions

Show all answers

1.

FLASHCARD QUESTION

Front

amino acid

Back

The subunit (monomer) molecule of a protein

2.

FLASHCARD QUESTION

Front

antibody/immunoglobulin

Back

Proteins made by the immune system in response to antigens

3.

FLASHCARD QUESTION

Front

antigen

Back

A substance that generally evokes an immune response in an organism when bound by an antibody

4.

FLASHCARD QUESTION

Front

assay

Back

A test to determine the quality or quantity of a sample

5.

FLASHCARD QUESTION

Front

bradford assay

Back

A protein identification technique that utilizes a non-specific protein binding dye

6.

FLASHCARD QUESTION

Front

column chromatography

Back

A method of separating molecules in a vertical chamber relying on gravity and molecular interaction

7.

FLASHCARD QUESTION

Front

denature

Back

When a protein loses its secondary structure (shape or conformation), the primary structure is not affected

Create a free account and access millions of resources

Create resources

Host any resource

Get auto-graded reports

Continue with Google

Continue with Email

Continue with Classlink

Continue with Clever

or continue with

Microsoft

Apple

Others

Already have an account?

Similar Resources on Wayground

42 questions

lesson 8

Flashcard

•

10th Grade

47 questions

Conservation Vocabulary

Flashcard

•

12th Grade

50 questions

CHAPTER 8 FLASHCARD LOVELIES SB025

Flashcard

•

University

45 questions

Contemporary Philippine Arts

Flashcard

•

12th Grade

37 questions

Queen Flash Cards

Flashcard

•

11th Grade

41 questions

Unit 6

Flashcard

•

University

50 questions

Vocabulaire de la fête

Flashcard

•

9th Grade

Popular Resources on Wayground

10 questions

Forest Self-Management

Lesson

•

1st - 5th Grade

25 questions

Multiplication Facts

Quiz

•

5th Grade

30 questions

Thanksgiving Trivia

Quiz

•

9th - 12th Grade

30 questions

Thanksgiving Trivia

Quiz

•

6th Grade

11 questions

Would You Rather - Thanksgiving

Lesson

•

KG - 12th Grade

48 questions

The Eagle Way

Quiz

•

6th Grade

10 questions

Identifying equations

Quiz

•

KG - University

10 questions

Thanksgiving

Lesson

•

5th - 7th Grade

Discover more resources for Biology

17 questions

Check: Energy pyramids and Water Cycles

Quiz

•

9th - 12th Grade

48 questions

CMS Biology Unit 3 Summative #2

Quiz

•

9th - 12th Grade

14 questions

Natural Selection

Quiz

•

9th - 12th Grade

10 questions

4-4 Active Transport

Quiz

•

9th - 12th Grade

20 questions

Blood Types

Quiz

•

9th - 12th Grade

38 questions

Evolution Review

Quiz

•

9th - 12th Grade