Smears, Gram Stain, Aseptic Transfer

Flashcard
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Biology
•
University
•
Hard
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15 questions
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1.
FLASHCARD QUESTION
Front
What is the secondary stain used in a Gram stain procedure?
Back
Safranin
2.
FLASHCARD QUESTION
Front
What is the critical step in the Gram staining procedure?
Back
The timing of the decolorizer
3.
FLASHCARD QUESTION
Front
What is the purpose of the mordant in Gram staining?
Back
Combines with the primary dye and helps stabilize it within the peptidoglycan.
4.
FLASHCARD QUESTION
Front
What type of bacteria stains purple in a Gram stain?
Back
Gram (+) bacteria.
5.
FLASHCARD QUESTION
Front
True or False: Bacteria that stain purple are considered gram negative.
Back
False.
6.
FLASHCARD QUESTION
Front
What is the primary stain used in a Gram stain procedure?
Back
Crystal violet.
7.
FLASHCARD QUESTION
Front
What role does iodine play in the Gram staining process?
Back
Iodine acts as a mordant, forming a complex with crystal violet to enhance staining.
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