Smears, Gram Stain, Aseptic Transfer

Front

What is the secondary stain used in a Gram stain procedure?

Back

Safranin

Front

What is the critical step in the Gram staining procedure?

Back

The timing of the decolorizer

Front

What is the purpose of the mordant in Gram staining?

Back

Combines with the primary dye and helps stabilize it within the peptidoglycan.

Front

What type of bacteria stains purple in a Gram stain?

Back

Gram (+) bacteria.

Front

True or False: Bacteria that stain purple are considered gram negative.

Back

False.

Front

What is the primary stain used in a Gram stain procedure?

Back

Crystal violet.

Front

What role does iodine play in the Gram staining process?

Back

Iodine acts as a mordant, forming a complex with crystal violet to enhance staining.

Front

What is the effect of acetone-alcohol in the Gram staining process?

Back

It acts as a decolorizer, differentiating between Gram-positive and Gram-negative bacteria.

Front

What color do Gram-negative bacteria appear after Gram staining?

Back

Pink.

Front

What is the structure that the Gram stain targets in bacteria?

Back

The peptidoglycan layer of the bacterial cell wall.