U3 AOS 1: Polymerase Chain Reaction (PCR)

Front

What is the purpose of Polymerase Chain Reaction (PCR)?

Back

To amplify DNA sequences.

Front

Name two applications of PCR.

Back

Paternity testing and forensic testing.

Front

What is used to analyze gene fragments in PCR?

Back

Primers and restriction endonucleases.

Front

What happens to DNA at 90℃ - 95℃ in PCR?

Back

Hydrogen bonds are broken, and DNA is denatured.

Front

What is the role of Taq polymerase in PCR?

Back

It synthesizes new DNA strands by adding complementary nucleotides.

Front

What is the function of a thermal cycler in PCR?

Back

It precisely controls the temperature changes required for denaturation, annealing, and elongation.

Front

At what temperature does annealing occur in PCR?

Back

50℃ - 55℃.

Front

At what temperature does elongation occur in PCR?

Back

72℃.

Front

What is the role of a forward primer in PCR?

Back

It binds to the 3’ end of the template strand.

Front

What is the role of a reverse primer in PCR?

Back

It binds to the 3’ end of the coding strand.