Gel Electrophoresis & DNA Fingerprinting

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What is gel electrophoresis?

Back

A laboratory technique used to separate DNA, RNA, or proteins based on their size and charge.

Front

What is the purpose of gel electrophoresis?

Back

To separate DNA fragments based on size.

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What does DNA fingerprinting refer to?

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A method used to identify individuals by analyzing the unique patterns in their DNA.

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Why is DNA fingerprinting effective?

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Because no two people, except identical twins, have exactly the same DNA.

Front

What is the role of agarose gel in gel electrophoresis?

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Agarose gel acts as a medium through which DNA fragments can move during electrophoresis.

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What is the charge of DNA molecules?

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DNA molecules are negatively charged due to their phosphate backbone.

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What is the significance of the size of DNA fragments in gel electrophoresis?

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Smaller DNA fragments move faster and farther through the gel than larger fragments.

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What is a DNA ladder in gel electrophoresis?

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A set of known DNA fragments used as a reference to determine the size of unknown DNA samples.

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What is the function of a loading dye in gel electrophoresis?

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Loading dye helps visualize the DNA samples and allows for tracking their movement during electrophoresis.

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What is the first step in DNA fingerprinting?

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The extraction of DNA from the sample.