MI 1.1 Review

Front

What is the Polymerase Chain Reaction (PCR)?

Back

A technique used to amplify small amounts of DNA to produce millions of copies.

Front

What are the steps of DNA Sequencing in order?

Back

1. PCR with fluorescent dideoxynucleotides 2. Separate DNA by size with gel electrophoresis 3. Detection of fluorescence with lasers & sensors 4. Match DNA sequence using BLAST database.

Front

What is the role of enzymes and substrates in an ELISA assay?

Back

They facilitate a color change to indicate the presence of a target substance.

Front

What do dideoxynucleotides do during Cycle Sequencing?

Back

They stop the elongation of the growing DNA strand.

Front

Which of the following is NOT a medical intervention? Toothbrush, Appendectomy, Physical therapy, None of the above

Back

None of the above.

Front

Define ELISA.

Back

Enzyme-Linked Immunosorbent Assay, a test used to detect and quantify proteins, antibodies, or hormones.

Front

What is gel electrophoresis used for?

Back

To separate DNA fragments based on their size.

Front

What is the purpose of using fluorescent dideoxynucleotides in DNA sequencing?

Back

To terminate DNA strand elongation at specific bases, allowing for sequence determination.

Front

What is the function of primary antibodies in an ELISA?

Back

To bind specifically to the target antigen.

Front

What is the function of secondary antibodies in an ELISA?

Back

To bind to the primary antibodies and often carry an enzyme for detection.