Fun Wheel

Front

In vitro studies are always directly applicable to human physiology.

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False - In vitro studies are done in controlled environments (like petri dishes or flasks) and lack the complexity of the whole human body. Results provide valuable insights but are not always directly applicable to human physiology without further validation in in vivo or clinical studies.

Front

One advantage of in vitro studies is reduced ethical concerns compared to animal models.

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True - uses isolated cells or tissues, which reduces the ethical concerns linked to animal testing. They follow the 3Rs principle (Replacement, Reduction, Refinement) and are often more acceptable in early-stage research before moving to higher range studies.

Front

Primary cultures have unlimited lifespan in vitro.

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False - After a few passages, the cells undergo senescence and stop dividing. Only continuous or immortalized cell lines can divide indefinitely in vitro.

Front

In vitro studies cannot be reproduced easily because the environment is uncontrolled.

Back

False - carried out under carefully controlled conditions (e.g., pH, nutrient levels, temperature, CO₂), which makes them highly reproducible.

Front

Cell culture requires aseptic conditions to prevent contamination.

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True - Cell culture work must be performed under aseptic conditions to prevent biological contamination. Even minor contamination can compromise results or destroy the culture.

Front

A biosafety cabinet class II provides protection for both the culture and the personnel.

Back

True - maintain a sterile environment for the cell culture while also protecting the researcher and the laboratory environment through laminar airflow and HEPA filtration. This makes them the standard choice for most cell culture work.

Front

All transformed cell lines stop dividing after a few passages.

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False - Transformed including continuous/immortalized cell lines do not stop dividing after a few passages. They can proliferate indefinitely due to genetic changes that bypass normal cellular senescence. It is the finite cell lines (derived from primary cultures) that eventually stop dividing after a limited number of passages.

Front

In vitro assays can be used to assess antioxidant capacity of foods.

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True - test such as DPPH, ABTS, and FRAP are widely used to measure the antioxidant capacity of foods, plant extracts, or bioactive compounds. These tests provide preliminary screening before moving into in vivo or clinical studies.

Front

BSL-1 biosafety cabinets offer protection to laboratory personnel and the environment, but they do not protect cultures from contamination.

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True - Class I cabinets pull air from the room into the cabinet and then exhaust it through HEPA filters to protect laboratory personnel and the environment. However, the incoming air is not filtered before passing over the culture, which means contaminants can enter the workspace and compromise sterility of the culture.

Front

Principle of in vitro study:

Results are often inconsistent because of controlled conditions.

Back

False - In vitro studies are carried out in carefully controlled laboratory conditions such as pH, nutrient concentration, temperature, and CO₂ levels. Because of this, experiments can be reproduced with high consistency, making reproducibility one of the major strengths of in vitro research compared to in vivo studies.