MI Lab 2.1.2 Flashcard

Front

What are the correct steps, in order, to perform PCR?

Back

denaturation, annealing, extension

Front

DNA Polymerase builds a new strand of DNA in the

Back

5' -----> 3' direction

Front

What are the four "free deoxynucleotides used in PCR?

Back

dATP, dCTP, dGTP, and dTTP

Front

PCR requires the use of a thermostable DNA polymerase, an enzyme. What is the most commonly used?

Back

Taq

Front

What are the four components of PCR?

Back

template DNA, primers, the four deoxynucleotides, and Taq DNA polymerase

Front

When the four components are added in PCR, they are mixed with a buffer and which one of the following?

Back

Mg+2

Front

Mathematically, PCR can be expressed as an exponential relationship - if we begin with a starting copy number of m, then after n cycles, we will have _______________.

Back

m x 2ⁿ copies of our DNA target

Front

What is one common problem that occurs during PCR?

Back

unwanted amplification products

Front

Medical diagnostics use PCR to identify __________ and __________.

Back

genetic mutations, infectious agents

Front

In the PCR lab, what does the reaction pellet or bead containing the dNTP mixture contain?

Back

Taq DNA polymerase and buffer, and MgCl2